PMID- 8387800
OWN - NLM
STAT- MEDLINE
DCOM- 19930616
LR  - 20190914
IS  - 0903-4641 (Print)
IS  - 0903-4641 (Linking)
VI  - 101
IP  - 2
DP  - 1993 Feb
TI  - Acridine orange-mediated photodamage to cultured cells.
PG  - 127-32
AB  - Photosensitization mediated by the lysosomotropic, weakly basic dye acridine 
      orange (AO) was studied on cultured J-774 cells. The phototoxicity was found to 
      be potentiated by elevated oxygen tension and reduced at low oxygen tension. 
      Moreover, cell cultures pre-exposed to the singlet oxygen scavenger sodium azide 
      showed pronounced protection against the loss of viability induced by AO and blue 
      light. AO-mediated photosensitization was neither increased by pre-exposure of 
      cell cultures to ferric chloride or the catalase-inhibitor aminotriazole nor 
      decreased by exposure to deferoxamine. These observations suggest that type II 
      (singlet oxygen-mediated) reactions predominate over type I reactions 
      (radical-mediated). A rapid and pronounced decrease in lysosomal cathepsin L 
      activity (up to 60%) was observed after an initial 10 min irradiation, indicating 
      the lysosomal compartment to be an early target. This irradiation time did not, 
      however, result in any substantial loss of viability. Levels of cytosolic lactate 
      dehydrogenase were unaffected even after 30 min irradiation, indicating that 
      neither cytosol nor plasma membrane is a primary target of the AO-mediated 
      photodamage. Glutathione depletion by pre-exposure to buthionine-S,R-sulfoximine 
      (BSO) much enhanced the sensitivity of J-774 cells to AO-mediated 
      photosensitization, indicating a protective role for thiol-containing compounds 
      against AO-mediated photodamage.
FAU - Zdolsek, J M
AU  - Zdolsek JM
AD  - Department of Pathology II, Faculty of Health Sciences, Linkoping University, 
      Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (Azides)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (Radiation-Sensitizing Agents)
RN  - 1982-67-8 (Methionine Sulfoximine)
RN  - 5072-26-4 (Buthionine Sulfoximine)
RN  - 968JJ8C9DV (Sodium Azide)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.15 (Cathepsin L)
RN  - EC 3.4.22.15 (Ctsl protein, mouse)
RN  - F30N4O6XVV (Acridine Orange)
RN  - GAN16C9B8O (Glutathione)
RN  - J06Y7MXW4D (Deferoxamine)
RN  - S88TT14065 (Oxygen)
RN  - ZF80H5GXUF (Amitrole)
SB  - IM
MH  - Acridine Orange/*pharmacology
MH  - Amitrole/pharmacology
MH  - Animals
MH  - Azides/pharmacology
MH  - Buthionine Sulfoximine
MH  - Cathepsin L
MH  - Cathepsins/physiology
MH  - Cell Death/physiology
MH  - Cysteine Endopeptidases
MH  - Cytosol/enzymology
MH  - Deferoxamine/pharmacology
MH  - *Endopeptidases
MH  - Glutathione/physiology
MH  - Histiocytoma, Benign Fibrous
MH  - L-Lactate Dehydrogenase/analysis
MH  - Light/*adverse effects
MH  - Lysosomes/enzymology/metabolism/ultrastructure
MH  - Methionine Sulfoximine/analogs & derivatives/pharmacology
MH  - Mice
MH  - Oxygen/pharmacology
MH  - Partial Pressure
MH  - Photosensitizing Agents/*pharmacology
MH  - Radiation-Sensitizing Agents/pharmacology
MH  - Sodium Azide
MH  - Tumor Cells, Cultured/*drug effects/*radiation effects
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
AID - 10.1111/j.1699-0463.1993.tb00091.x [doi]
PST - ppublish
SO  - APMIS. 1993 Feb;101(2):127-32. doi: 10.1111/j.1699-0463.1993.tb00091.x.