PMID- 8388815
OWN - NLM
STAT- MEDLINE
DCOM- 19930628
LR  - 20131121
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 157
IP  - 2
DP  - 1993 Jun
TI  - Expression of the met/hepatocyte growth factor/scatter factor receptor and its 
      ligand during differentiation of murine P19 embryonal carcinoma cells.
PG  - 308-20
AB  - The met proto-oncogene is a member of the family of tyrosine kinase growth factor 
      receptors and was recently identified as a receptor for hepatocyte growth factor 
      and scatter factor (HGF/SF). From Northern hybridization studies the met/HGF/SF 
      receptor (R) is expressed in many adult and embryonic mouse tissues. To identify 
      which specific differentiated cell types express the met/HGF/SFR and to 
      investigate the biological function of this receptor and its ligand during early 
      murine development, we chose to study the expression of the met/HGF/SFR and 
      HGF/SF during differentiation of the pluripotent P19 murine embryonal carcinoma 
      cell line in culture. In this paper we demonstrate that met/HGF/SFR mRNA, 
      protein, and its ligand are expressed at a low level in undifferentiated P19 
      cells and that their expression is increased as P19 cells are induced to 
      differentiate into neuroectodermal derivatives following treatment with retinoic 
      acid (RA) and into mesodermal derivatives following treatment with dimethyl 
      sulfoxide (DMSO). From in situ hybridization analyses, only a subpopulation of 
      differentiating P19 cells treated with RA or DMSO expresses high levels of the 
      met/HGF/SFR RNA. In cultures treated with RA and cytosine arabinoside, both 
      met/HGF/SFR mRNA and protein can be localized specifically to nondividing 
      neuronal cells. Expression of the met/HGF/SFR and its ligand, HGF/SF, in 
      undifferentiated P19 cells and differentiated derivatives suggests that 
      stimulation of this signal transduction pathway may be an important event for the 
      control of cell differentiation, proliferation, or positioning during 
      embryogenesis.
FAU - Yang, X M
AU  - Yang XM
AD  - Department of Oncology, Royal Victoria Hospital, Montreal, Quebec, Canada.
FAU - Park, M
AU  - Park M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Drug Combinations)
RN  - 0 (Ligands)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 04079A1RDZ (Cytarabine)
RN  - 5688UTC01R (Tretinoin)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Differentiation/drug effects
MH  - Cytarabine/pharmacology
MH  - Dimethyl Sulfoxide/pharmacology
MH  - Drug Combinations
MH  - Ectoderm
MH  - Embryonal Carcinoma Stem Cells
MH  - Ligands
MH  - Mesoderm
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neoplastic Stem Cells/drug effects/*metabolism
MH  - Neurons/*metabolism
MH  - Proto-Oncogene Proteins/analysis/*metabolism
MH  - Proto-Oncogene Proteins c-met
MH  - RNA, Messenger/analysis
MH  - Receptors, Cell Surface/*metabolism
MH  - Signal Transduction
MH  - Tretinoin/pharmacology
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - S0012-1606(83)71137-1 [pii]
AID - 10.1006/dbio.1993.1137 [doi]
PST - ppublish
SO  - Dev Biol. 1993 Jun;157(2):308-20. doi: 10.1006/dbio.1993.1137.