PMID- 8388878
OWN - NLM
STAT- MEDLINE
DCOM- 19930629
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 121
IP  - 5
DP  - 1993 Jun
TI  - Cell cycle-dependent specific positioning and clustering of centromeres and 
      telomeres in fission yeast.
PG  - 961-76
AB  - Fluorescence in situ hybridization (FISH) shows that fission yeast centromeres 
      and telomeres make up specific spatial arrangements in the nucleus. Their 
      positioning and clustering are cell cycle regulated. In G2, centromeres cluster 
      adjacent to the spindle pole body (SPB), while in mitosis, their association with 
      each other and with the SPB is disrupted. Similarly, telomeres cluster at the 
      nuclear periphery in G2 and their associations are disrupted in mitosis. Mitotic 
      centromeres interact with the spindle. They remain undivided until the spindle 
      reaches a critical length, then separate and move towards the poles. This 
      demonstrated, for the first time, that anaphase A occurs in fission yeast. The 
      mode of anaphase A and B is similar to that of higher eukaryotes. In nda3 and 
      cut7 mutants defective in tubulin of a kinesin-related motor, cells are blocked 
      in early stages of mitosis due to the absence of the spindle, and centromeres 
      dissociate but remain close to the SPB, whereas in a metaphase-arrested nuc2 
      mutant, they reside at the middle of the spindle. FISH is therefore a powerful 
      tool for analyzing mitotic chromosome movement and disjunction using various 
      mutants. Surprisingly, in top2 defective in DNA topoisomerase II, while most 
      chromatid DNAs remain undivided, sister centromeres are separated. Significance 
      of this finding is discussed. In contrast, most chromatid DNAs are separated but 
      telomeric DNAs are not in cut1 mutant. In cut1, the dependence of SPB duplication 
      on the completion of mitosis is abolished. In crm1 mutant cells defective in 
      higher-order chromosome organization, the interphase arrangements of centromeres 
      and telomeres are disrupted.
FAU - Funabiki, H
AU  - Funabiki H
AD  - Department of Biophysics, Faculty of Science, Kyoto University, Japan.
FAU - Hagan, I
AU  - Hagan I
FAU - Uzawa, S
AU  - Uzawa S
FAU - Yanagida, M
AU  - Yanagida M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - EC 5.99.1.3 (DNA Topoisomerases, Type II)
SB  - IM
GS  - cdc25
GS  - crm1
GS  - cut7
GS  - nda3
GS  - nuc2
MH  - *Cell Cycle
MH  - Cell Nucleus/*ultrastructure
MH  - Centromere/*ultrastructure
MH  - DNA Topoisomerases, Type II/physiology
MH  - Fluorescent Antibody Technique
MH  - In Situ Hybridization
MH  - Interphase
MH  - Mitosis
MH  - Schizosaccharomyces/*ultrastructure
MH  - Spindle Apparatus/ultrastructure
MH  - Telomere/*ultrastructure
PMC - PMC2119680
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
PMCR- 1993/12/01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
PHST- 1993/12/01 00:00 [pmc-release]
AID - 93273802 [pii]
AID - 10.1083/jcb.121.5.961 [doi]
PST - ppublish
SO  - J Cell Biol. 1993 Jun;121(5):961-76. doi: 10.1083/jcb.121.5.961.