PMID- 8389029
OWN - NLM
STAT- MEDLINE
DCOM- 19930629
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 8
IP  - 6
DP  - 1993 Jun
TI  - Sublocalization of the synovial sarcoma-associated t(X;18) chromosomal breakpoint 
      in Xp11.2 using cosmid cloning and fluorescence in situ hybridization.
PG  - 1457-63
AB  - In a previous study we localized the synovial sarcoma-associated t(X;18)(p11;q11) 
      breakpoint within the ornithine aminotransferase-like 1 (OATL1) cluster on the X 
      chromosome. This localization was delineated from both somatic cell hybrid and 
      fluorescence in situ hybridization (FISH) analysis of patient material, using 
      OAT-specific cDNA and YAC probes. Simultaneously, Knight et al. (1992, Mol. Hum. 
      Genet, in press) mapped this same breakpoint in their patient material adjacent 
      to the more proximal OATL2 region on the X chromosome. Here we report the 
      analysis of two additional tumors and demonstrate that again in these cases the 
      chromosomal break occurs within the OATL1 cluster. In order to further specify 
      the breakpoint, we subcloned the OATL1 YAC (no. 2) into cosmids. At least one of 
      these cosmids (0.38) hybridizes to sequences that bracket the translocation 
      breakpoint, as demonstrated by both Southern blot and FISH analysis. These 
      observations confirm and substantiate our previous findings. In addition, cosmid 
      0.38 should be a valuable instrument for the ultimate isolation and 
      identification of the gene(s) involved in the development of synovial sarcoma.
FAU - de Leeuw, B
AU  - de Leeuw B
AD  - Department of Human Genetics, University Hospital, Nijmegen, The Netherlands.
FAU - Suijkerbuijk, R F
AU  - Suijkerbuijk RF
FAU - Balemans, M
AU  - Balemans M
FAU - Sinke, R J
AU  - Sinke RJ
FAU - de Jong, B
AU  - de Jong B
FAU - Molenaar, W M
AU  - Molenaar WM
FAU - Meloni, A M
AU  - Meloni AM
FAU - Sandberg, A A
AU  - Sandberg AA
FAU - Geraghty, M
AU  - Geraghty M
FAU - Hofker, M
AU  - Hofker M
AU  - et al.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA, Neoplasm)
RN  - EC 2.6.1.13 (Ornithine-Oxo-Acid Transaminase)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Cell Line
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 18
MH  - Cloning, Molecular
MH  - Cosmids
MH  - Cricetinae
MH  - Cricetulus
MH  - DNA, Neoplasm/genetics/isolation & purification
MH  - Humans
MH  - Hybrid Cells/cytology
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Multigene Family
MH  - Ornithine-Oxo-Acid Transaminase/genetics
MH  - Restriction Mapping
MH  - Sarcoma, Synovial/*genetics
MH  - *Translocation, Genetic
MH  - *X Chromosome
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1993 Jun;8(6):1457-63.