PMID- 8389192
OWN - NLM
STAT- MEDLINE
DCOM- 19930708
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 32
IP  - 22
DP  - 1993 Jun 8
TI  - The human erythrocyte inflammatory peptide (chemokine) receptor. Biochemical 
      characterization, solubilization, and development of a binding assay for the 
      soluble receptor.
PG  - 5733-8
AB  - In addition to the two human interleukin-8 (IL-8) receptors that have been 
      cloned, IL-8RA and IL-8RB, we recently described a binding protein in human 
      erythrocytes that binds IL-8 and monocyte chemotactic peptide-1 (MCP-1), which we 
      have termed the chemokine (CK) receptor. This communication describes the 
      biochemical characterization, detergent solubilization, and development of a 
      solubilized receptor binding assay for the erythrocyte CK receptor. Competitive 
      125I-IL-8 binding studies in cells transfected with IL-8RA and IL-8RB revealed 
      that only IL-8 and MGSA were able to displace the radiolabeled IL-8 from these 
      cells. In contrast, a whole array of chemokines were able to cross-compete with 
      125I-IL-8 for binding to the CK receptor in erythrocyte ghosts. Scatchard 
      analysis of 125I-IL-8 binding to erythrocyte membranes and to dodecyl 
      beta-maltoside solubilized CK receptors revealed a single class of high affinity 
      binding sites in both cases with KD values of 9.5 nM +/- 3.6 and 15.4 nM +/- 5.0, 
      respectively. Chemical cross-linking studies with erythrocyte membranes and with 
      solubilized CK receptors indicated that the CK receptor has a lower molecular 
      mass than the cloned IL-8 receptors (39 kDa compared to 57-69 kDa). Treatment of 
      the cross-linked 47-kDA protein with N-glycanase reduced its molecular mass to 42 
      kDa.
FAU - Horuk, R
AU  - Horuk R
AD  - Department of Protein Chemistry, Genentech, Inc., South San Francisco, California 
      94080.
FAU - Colby, T J
AU  - Colby TJ
FAU - Darbonne, W C
AU  - Darbonne WC
FAU - Schall, T J
AU  - Schall TJ
FAU - Neote, K
AU  - Neote K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (CXCL1 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Detergents)
RN  - 0 (Growth Substances)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-8)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Interleukin-8A)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.5.- (Amidohydrolases)
RN  - EC 3.5.1.52 (Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase)
SB  - IM
MH  - Amidohydrolases/pharmacology
MH  - Binding, Competitive
MH  - Chemokine CCL2
MH  - Chemokine CXCL1
MH  - *Chemokines, CXC
MH  - Chemotactic Factors/*blood/metabolism
MH  - Detergents
MH  - Erythrocyte Membrane/drug effects/*metabolism
MH  - Growth Substances/metabolism
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Guanosine Triphosphate/pharmacology
MH  - Humans
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Interleukin-8/*blood
MH  - Molecular Weight
MH  - Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
MH  - Receptors, Cell Surface/chemistry/*metabolism
MH  - Receptors, Immunologic/genetics/metabolism
MH  - Receptors, Interleukin-8A
MH  - Solubility
MH  - Transfection
EDAT- 1993/06/08 00:00
MHDA- 1993/06/08 00:01
CRDT- 1993/06/08 00:00
PHST- 1993/06/08 00:00 [pubmed]
PHST- 1993/06/08 00:01 [medline]
PHST- 1993/06/08 00:00 [entrez]
AID - 10.1021/bi00073a002 [doi]
PST - ppublish
SO  - Biochemistry. 1993 Jun 8;32(22):5733-8. doi: 10.1021/bi00073a002.