PMID- 8389460
OWN - NLM
STAT- MEDLINE
DCOM- 19930707
LR  - 20191210
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 90
IP  - 11
DP  - 1993 Jun 1
TI  - Intracellular Ca2+ pool content is linked to control of cell growth.
PG  - 4986-90
AB  - A close correlation was observed between intracellular Ca2+ pool depletion and 
      refilling and the onset of DNA synthesis and proliferation of DDT1MF-2 smooth 
      muscle cells. The intracellular Ca2+ pump inhibitors 
      2,5-di-tert-butyl-hydroquinone (DBHQ) and thapsigargin (TG) specifically emptied 
      identical inositol 1,4,5-trisphosphate (InsP3)-sensitive Ca2+ pools and both 
      arrested cell growth at concentrations corresponding to Ca2+ pump blockade. 
      However, an important distinction was observed between the two inhibitors with 
      respect to their reversibility of action. Upon removal of DBHQ from DBHQ-arrested 
      cells, Ca2+ pools immediately refilled, and 14 hr later cells entered S phase 
      followed by normal cell proliferation; the time for entry into S phase was 
      identical to that for cells released from confluence arrest. Although TG 
      irreversibly blocked Ca2+ pumping and emptied Ca2+ pools, high serum treatment of 
      TG-arrested cells induced recovery of functional Ca2+ pools in 6 hr (via probable 
      synthesis of new pump); thereafter cells proceeded to S phase and normal cell 
      proliferation within the same time period (14 hr) as that following release of 
      DBHQ-arrested cells. The precise relationship between Ca2+ pump blockade and 
      growth arrest indicates that Ca2+ pool emptying maintains cells in a G0-like 
      quiescent state; upon refilling of pools, normal progression into the cell cycle 
      is resumed. It is possible that a specific cell cycle event necessary for G0 to 
      G1 transition depends upon signals generated from the InsP3-sensitive Ca2+ pool.
FAU - Short, A D
AU  - Short AD
AD  - Department of Biological Chemistry, University of Maryland School of Medicine, 
      Baltimore 21201.
FAU - Bian, J
AU  - Bian J
FAU - Ghosh, T K
AU  - Ghosh TK
FAU - Waldron, R T
AU  - Waldron RT
FAU - Rybak, S L
AU  - Rybak SL
FAU - Gill, D L
AU  - Gill DL
LA  - eng
GR  - NS19304/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antioxidants)
RN  - 0 (Hydroquinones)
RN  - 0 (Terpenes)
RN  - 56092-81-0 (Ionomycin)
RN  - 67526-95-8 (Thapsigargin)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - C12674942B (2-tert-butylhydroquinone)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/antagonists & inhibitors/drug effects/*physiology
MH  - Cell Division/drug effects/*physiology
MH  - Cell Line
MH  - Fura-2
MH  - Hydroquinones/pharmacology
MH  - Inositol 1,4,5-Trisphosphate/metabolism
MH  - Ionomycin/pharmacology
MH  - Kinetics
MH  - Muscle, Smooth
MH  - Spectrometry, Fluorescence
MH  - Terpenes/pharmacology
MH  - Thapsigargin
MH  - Time Factors
PMC - PMC46638
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
PMCR- 1993/12/01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
PHST- 1993/12/01 00:00 [pmc-release]
AID - 10.1073/pnas.90.11.4986 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):4986-90. doi: 
      10.1073/pnas.90.11.4986.