PMID- 8391023
OWN - NLM
STAT- MEDLINE
DCOM- 19930728
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 31
IP  - 6
DP  - 1993 Jun
TI  - Prevalence of human herpesvirus 6 variant A and B infections in bone marrow 
      transplant recipients as determined by polymerase chain reaction and 
      sequence-specific oligonucleotide probe hybridization.
PG  - 1515-20
AB  - An oligotyping methodology was devised by using the polymerase chain reaction and 
      sequence-specific oligonucleotide probe hybridization in order to discriminate 
      the A and B variants of human herpesvirus 6 (HHV-6). Comparative DNA sequence 
      analysis of portions of the U1102 (variant A) and Z29 (variant B) genomes 
      revealed polymorphic regions which allowed for the synthesis of variant-specific 
      and consensus oligonucleotide probes. These probes were found to hybridize 
      exclusively to their respective HHV-6 variants. This strategy was then further 
      tested by evaluating 16 clinical isolates derived from patients undergoing bone 
      marrow transplantation to determine the subtype prevalence of HHV-6 infection in 
      these patients. All clinical isolates were documented to be of variant B, 
      indicating that the majority of bone marrow transplantation patients may be 
      preferentially infected with this HHV-6 subtype. This oligotyping strategy may be 
      useful in defining the relative prevalence of HHV-6A and HHV-6B infections in 
      patient populations potentially at risk for HHV-6 disease.
FAU - Drobyski, W R
AU  - Drobyski WR
AD  - Department of Medicine, Medical College of Wisconsin, Milwaukee.
FAU - Eberle, M
AU  - Eberle M
FAU - Majewski, D
AU  - Majewski D
FAU - Baxter-Lowe, L A
AU  - Baxter-Lowe LA
LA  - eng
SI  - GENBANK/L06127
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Bone Marrow Transplantation/*adverse effects
MH  - Child
MH  - DNA, Viral/genetics
MH  - Female
MH  - Genetic Variation
MH  - Herpesviridae Infections/classification/*etiology/microbiology
MH  - Herpesvirus 6, Human/classification/*genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotide Probes
MH  - Polymerase Chain Reaction
MH  - Sequence Homology, Nucleic Acid
PMC - PMC265570
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
PMCR- 1993/06/01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
PHST- 1993/06/01 00:00 [pmc-release]
AID - 10.1128/jcm.31.6.1515-1520.1993 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1993 Jun;31(6):1515-20. doi: 10.1128/jcm.31.6.1515-1520.1993.