PMID- 8392089
OWN - NLM
STAT- MEDLINE
DCOM- 19930812
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 92
IP  - 1
DP  - 1993 Jul
TI  - Chrysotile asbestos upregulates gene expression and production of alpha-receptors 
      for platelet-derived growth factor (PDGF-AA) on rat lung fibroblasts.
PG  - 425-30
AB  - PDGF isoforms have been postulated to serve as mediators of fibroblast 
      proliferation and chemotaxis during lung fibrogenesis induced by asbestos 
      inhalation. We have studied the interaction of chrysotile asbestos fibers with 
      rat lung fibroblasts (RLF) in vitro and the consequent changes in PDGF receptor 
      mRNA expression, PDGF binding, and mitogenic activity of PDGF isoforms. Northern 
      blot analysis revealed that mRNA for the PDGF-receptor alpha subtype (PDGF-R 
      alpha) on RLF was upregulated after a 24-h exposure to asbestos in culture 
      (0.5-15 micrograms fibers/cm2). [125I]PDGF-BB receptor assays showed that normal 
      RLF possess mainly PDGF-R beta and a paucity of PDGF-R alpha. In agreement with 
      the Northern data, saturation binding of [125I]PDGF-BB to RLF exposed to asbestos 
      demonstrated an approximately 40% increase in binding sites accompanied by a 
      twofold decrease in receptor affinity. Treating asbestos-exposed RLF with 
      PDGF-AA, which binds only PDGF-R alpha, blocked the PDGF binding sites that were 
      upregulated by fiber exposure. PDGF-AA had increased mitogenic potency for 
      fiber-exposed RLF, but PDGF-BB was a less potent mitogen for these RLF. 
      Nonfibrogenic carbonyl iron spheres induced similar changes in PDGF growth 
      responses. These data show that inorganic particulates alter the PDGF-R alpha 
      population on RLF without significant change in PDGF-R beta.
FAU - Bonner, J C
AU  - Bonner JC
AD  - Laboratory of Pulmonary Pathobiology, National Institute of Environmental Health 
      Sciences, Research Triangle Park, North Carolina 27709.
FAU - Goodell, A L
AU  - Goodell AL
FAU - Coin, P G
AU  - Coin PG
FAU - Brody, A R
AU  - Brody AR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Asbestos, Serpentine)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (RNA, Messenger)
RN  - 1332-21-4 (Asbestos)
RN  - EC 2.7.10.1 (Receptors, Platelet-Derived Growth Factor)
SB  - IM
MH  - Animals
MH  - Asbestos/*pharmacology
MH  - Asbestos, Serpentine
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Fibroblasts
MH  - Gene Expression/drug effects
MH  - In Vitro Techniques
MH  - Lung/cytology/*metabolism
MH  - Platelet-Derived Growth Factor/classification/*metabolism
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Receptors, Platelet-Derived Growth Factor/classification/*genetics/metabolism
MH  - Up-Regulation
PMC - PMC293628
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
PMCR- 1993/07/01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
PHST- 1993/07/01 00:00 [pmc-release]
AID - 10.1172/JCI116584 [doi]
PST - ppublish
SO  - J Clin Invest. 1993 Jul;92(1):425-30. doi: 10.1172/JCI116584.