PMID- 8392647
OWN - NLM
STAT- MEDLINE
DCOM- 19930816
LR  - 20161123
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 54
IP  - 4
DP  - 1993 Apr
TI  - Hypoxia induces a human macrophage cell line to release tumor necrosis 
      factor-alpha and its soluble receptors in vitro.
PG  - 281-5
AB  - Tissue hypoxia following hemorrhage and trauma is a possible initiating factor of 
      the generalized inflammatory response seen after shock. The role of hypoxia in 
      the release from a human monocyte cell line (THP-1) of tumor necrosis 
      factor-alpha (TNF alpha) and its soluble membrane receptors (TNF alpha R) 
      in-vitro is investigated in this study. Flat-bottom plates with 500,000 THP-1 
      cells/ml were placed in air-tight sealed boxes and exposed to hypoxia (O2 = 1%) 
      or controls (O2 = 9%) for up to 24 hr. Supernatants were tested for TNF alpha, as 
      well as 55- and 75-kDa soluble receptors for TNF alpha, by ELISA. Cell viability 
      was assessed by vital dye uptake and was found to be maintained throughout 
      hypoxic exposure. Medium pH levels were within normal range. In eight experiments 
      conducted in duplicate, minimal change over 24 hr occurred in control samples. 
      Control mean and SD were: TNF alpha = 12.0 +/- 4.2, 55-kDa R = 34.6 +/- 2.03, and 
      75-kDa R = 38.88 +/- 9.68 pg/ml. During hypoxia, TNF alpha was released as early 
      as the first 30 min of exposure (41.3 +/- 2.3 pg/ml) with a small peak at 1 hr 
      (52 +/- 5.0 pg/ml) and a later more pronounced peak at 18 hr (526 +/- 48 pg/ml). 
      Both 55- and 75-kDa R were released by the hypoxic monocytes; release was 
      progressive and was maximal at 24 hr in this study. Maximal release value of 
      55-kDa R was 236 +/- 15 pg/ml, while for 75-kDa R it was 2450 +/- 63 
      pg/ml.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Scannell, G
AU  - Scannell G
AD  - Department of Surgery, University of California, Irvine 92717.
FAU - Waxman, K
AU  - Waxman K
FAU - Kaml, G J
AU  - Kaml GJ
FAU - Ioli, G
AU  - Ioli G
FAU - Gatanaga, T
AU  - Gatanaga T
FAU - Yamamoto, R
AU  - Yamamoto R
FAU - Granger, G A
AU  - Granger GA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Capillaries
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Hypoxia/*metabolism/pathology
MH  - Macrophages/*metabolism
MH  - Osmolar Concentration
MH  - Oxygen/blood/pharmacology
MH  - Receptors, Cell Surface/chemistry/*metabolism
MH  - Receptors, Tumor Necrosis Factor
MH  - Solubility
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1993/04/01 00:00
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PHST- 1993/04/01 00:00 [pubmed]
PHST- 1993/04/01 00:01 [medline]
PHST- 1993/04/01 00:00 [entrez]
AID - S0022-4804(83)71044-9 [pii]
AID - 10.1006/jsre.1993.1044 [doi]
PST - ppublish
SO  - J Surg Res. 1993 Apr;54(4):281-5. doi: 10.1006/jsre.1993.1044.