PMID- 8392705
OWN - NLM
STAT- MEDLINE
DCOM- 19930813
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 21
IP  - 11
DP  - 1993 Jun 11
TI  - The glucocorticoid receptor hormone binding domain mediates transcriptional 
      activation in vitro in the absence of ligand.
PG  - 2673-81
AB  - We show that recombinant rat glucocorticoid receptor (vvGR) expressed using 
      vaccinia virus is indistinguishable from authentic GR with respect to DNA and 
      hormone binding. In the absence of hormone, vvGR is mainly found in the cytoplasm 
      in a complex with heat shock protein 90. Upon incubation with ligand, vvGR is 
      released from this complex and translocated to the nucleus. Thus, the ligand 
      binding domain displays the known biochemical properties. However, in vitro, 
      transcription from a synthetic promoter and from the mouse mammary tumor virus 
      (MMTV) promoter is enhanced by recombinant GR in a ligand independent manner. 
      Both transactivation domains contribute to the transcriptional activity, 
      additively on a synthetic promoter and cooperatively on the MMTV promoter. We 
      thus provide the first evidence that in vitro the hormone binding domain has a 
      transcriptional activity even in the absence of ligand.
FAU - Schmitt, J
AU  - Schmitt J
AD  - European Molecular Biology Laboratory, Gene Expression Programme, Heidelberg, 
      Germany.
FAU - Stunnenberg, H G
AU  - Stunnenberg HG
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Recombinant Proteins)
RN  - F446C597KA (Triamcinolone Acetonide)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA-Binding Proteins/isolation & purification/*metabolism
MH  - Gene Expression
MH  - HeLa Cells
MH  - Humans
MH  - Kinetics
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides/*metabolism
MH  - *Promoter Regions, Genetic
MH  - Rats
MH  - Receptors, Glucocorticoid/isolation & purification/*metabolism
MH  - Recombinant Proteins/isolation & purification/metabolism
MH  - Templates, Genetic
MH  - *Transcription, Genetic
MH  - Transfection
MH  - Triamcinolone Acetonide/*metabolism
MH  - Vaccinia virus/genetics
PMC - PMC309598
EDAT- 1993/06/11 00:00
MHDA- 1993/06/11 00:01
CRDT- 1993/06/11 00:00
PHST- 1993/06/11 00:00 [pubmed]
PHST- 1993/06/11 00:01 [medline]
PHST- 1993/06/11 00:00 [entrez]
AID - 10.1093/nar/21.11.2673 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1993 Jun 11;21(11):2673-81. doi: 10.1093/nar/21.11.2673.