PMID- 8392808
OWN - NLM
STAT- MEDLINE
DCOM- 19930813
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 264
IP  - 6 Pt 1
DP  - 1993 Jun
TI  - 11 beta-hydroxysteroid dehydrogenase and corticosteroid hormone receptors in the 
      rat colon.
PG  - E951-7
AB  - In the rat kidney 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) maintains 
      normal in vivo specificity for mineralocorticoid receptor (MR) by converting the 
      active steroid corticosterone to inactive 11-dehydrocorticosterone, leaving 
      aldosterone to occupy the MR. Clinical observations support the hypothesis that 
      11 beta-HSD also protects the distal colonic MR from glucocorticoid excess. We 
      have measured 11 beta-HSD mRNA and activity along the rat colon and have analyzed 
      the distribution of 11 beta-HSD, MR, and glucocorticoid receptor (GR) mRNA within 
      rat distal colon using in situ hybridization. Levels of 11 beta-HSD mRNA (1.7 and 
      3.4 kb) and activity were higher in distal vs. proximal colon, paralleling 
      reported MR mRNA levels. Within the distal colon mucosa both 11 beta-HSD 
      immunoreactivity and mRNA was observed in cells in the lamina propria but not in 
      epithelial cells. MR mRNA was present in surface epithelial cells, but was also 
      colocalized with the same 11 beta-HSD-expressing cells in the lamina propria. In 
      contrast GR mRNA was more uniformly distributed. The localization of MR mRNA to 
      nonepithelial cells in the lamina propria, possibly neuroendocrine cells, 
      suggests that mineralocorticoid-regulated sodium transport across colonic 
      epithelial cells may also involve a paracrine mechanism. As with the kidney, 
      exposure of active mineralocorticoid to the MR in these cells in the lamina 
      propria is dictated by 11 beta-HSD in an autocrine fashion.
FAU - Whorwood, C B
AU  - Whorwood CB
AD  - Department of Medicine, Queen Elizabeth Hospital, University of Birmingham, 
      Edgbaston, United Kingdom.
FAU - Barber, P C
AU  - Barber PC
FAU - Gregory, J
AU  - Gregory J
FAU - Sheppard, M C
AU  - Sheppard MC
FAU - Stewart, P M
AU  - Stewart PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Mineralocorticoid)
RN  - 0 (Receptors, Steroid)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Animals
MH  - Colon/*metabolism
MH  - Hydroxysteroid Dehydrogenases/genetics/*metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cell Surface/*metabolism
MH  - Receptors, Glucocorticoid/genetics/*metabolism
MH  - Receptors, Mineralocorticoid
MH  - Receptors, Steroid/genetics/*metabolism
MH  - Tissue Distribution
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - 10.1152/ajpendo.1993.264.6.E951 [doi]
PST - ppublish
SO  - Am J Physiol. 1993 Jun;264(6 Pt 1):E951-7. doi: 10.1152/ajpendo.1993.264.6.E951.