PMID- 8392935
OWN - NLM
STAT- MEDLINE
DCOM- 19930820
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 12
IP  - 7
DP  - 1993 Jul
TI  - Thrombospondin related anonymous protein (TRAP) of Plasmodium falciparum binds 
      specifically to sulfated glycoconjugates and to HepG2 hepatoma cells suggesting a 
      role for this molecule in sporozoite invasion of hepatocytes.
PG  - 2881-9
AB  - Thrombospondin related anonymous protein (TRAP) of Plasmodium falciparum contains 
      an amino acid motif based around the sequence WSPCSVTCG which is also found in 
      region II of the circumsporozoite (CS) proteins of different species of 
      Plasmodium. This amino acid motif confers on the CS protein the ability to bind 
      specifically to sulfated glycoconjugates and to hepatocytes. This suggests that 
      the interaction of CS protein with sulfated glycoconjugates on the surface of the 
      hepatocytes may represent the first molecular event of sporozoite invasion of 
      liver cells. Experimental evidence indicates that TRAP is localized both on the 
      micronemes and on the surface of P. falciparum sporozoites implying that TRAP 
      with its putative sulfated glycoconjugate binding motif may also be involved in 
      recognition and/or entry of hepatocytes by the sporozoite. We show here that 
      different TRAP constructs expressed in Escherichia coli bind to 
      sulfogalactosyl-cerebrosides (sulfatides) and to the surface of HepG2 cells. 
      These interactions are dependent on the presence of the conserved amino acid 
      motif WSPCSVTCG within the sequences of the constructs and are completely 
      inhibited by several sulfated glycoconjugates as well as by suramin, a 
      polysulfonated drug with anti-protozoan activity. Moreover, sporozoite invasion 
      of HepG2 cells is inhibited by antisera raised against these different TRAP 
      constructs and by the presence of low concentrations of suramin. We concluded 
      that TRAP may be one of the parasite encoded molecules in the host-parasite 
      interaction that results in sporozoite invasion of hepatocytes.
FAU - Muller, H M
AU  - Muller HM
AD  - Istituto di Parassitologia, Universita di Roma, La Sapienza, P.A. Moro, Italy.
FAU - Reckmann, I
AU  - Reckmann I
FAU - Hollingdale, M R
AU  - Hollingdale MR
FAU - Bujard, H
AU  - Bujard H
FAU - Robson, K J
AU  - Robson KJ
FAU - Crisanti, A
AU  - Crisanti A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Glycoconjugates)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Sulfuric Acids)
RN  - 0 (Thrombospondins)
RN  - 120300-02-9 (thrombospondin-related adhesive protein, protozoan)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Carcinoma, Hepatocellular
MH  - Cloning, Molecular
MH  - DNA, Single-Stranded
MH  - Escherichia coli
MH  - Glycoconjugates/chemistry/*metabolism
MH  - Liver/*parasitology
MH  - Molecular Sequence Data
MH  - Plasmodium falciparum/*metabolism/physiology
MH  - Platelet Membrane Glycoproteins/genetics/metabolism
MH  - Protozoan Proteins/chemistry/genetics/*metabolism
MH  - Sulfuric Acids/chemistry
MH  - Thrombospondins
MH  - Tumor Cells, Cultured
PMC - PMC413541
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
PMCR- 1994/07/01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
PHST- 1994/07/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1993.tb05950.x [doi]
PST - ppublish
SO  - EMBO J. 1993 Jul;12(7):2881-9. doi: 10.1002/j.1460-2075.1993.tb05950.x.