PMID- 8393292
OWN - NLM
STAT- MEDLINE
DCOM- 19930826
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 265
IP  - 1 Pt 1
DP  - 1993 Jul
TI  - Bioactive-to-immunoreactive ACTH activity changes with severity of stress in 
      late-gestation ovine fetus.
PG  - E68-73
AB  - The late-gestation ovine fetus (> 130 days, 0.90 gestation, full term 145 days) 
      is capable of increased pituitary-adrenal activity following a variety of 
      stressors. To examine the relationship between plasma immunoreactive 
      adrenocorticotropic hormone (iACTH) and bioactive ACTH-like activity (bACTH), 
      late-gestation fetuses were studied under nonstress (group I, chronically 
      catheterized), "mild" stress (group II, acutely exteriorized), or marked stress 
      (group III, acutely exteriorized and hemorrhaged 30% of blood volume). Plasma 
      iACTH was determined by standard radioimmunoassay. Plasma bACTH was determined by 
      a bioassay that utilized dispersed rat adrenal cells. Plasma iACTH was lower in 
      group I than in groups II or III (78.4 +/- 16.3 vs. 320 +/- 116 and 622 +/- 144 
      pg/ml, respectively, mean +/- SE, P < 0.05 group I vs. III). Plasma bACTH was 
      significantly lower in groups I and II than in group III (13.8 +/- 2.7 and 52 +/- 
      22 vs. 601 +/- 106 pg/ml, respectively, P < 0.05). The ratio of bACTH to iACTH 
      was low in groups I and II but elevated in group III (0.188 +/- 0.028 and 0.091 
      +/- 0.07 vs. 0.996 +/- 0.122, P < 0.05). We conclude that the amount of bACTH 
      compared with iACTH in the late-gestation fetus is low under nonstress states or 
      mild stress and that bACTH increases disproportionately compared with iACTH after 
      marked stress. This suggests that a change in ACTH processing and/or secretion is 
      associated with stimulus intensity in the late-gestation ovine fetus.
FAU - Castro, M I
AU  - Castro MI
AD  - Department of Obstetrics and Gynecology, Bowman Gray School of Medicine, Wake 
      Forest University, Winston-Salem, North Carolina 27157-1066.
FAU - Valego, N K
AU  - Valego NK
FAU - Zehnder, T J
AU  - Zehnder TJ
FAU - Rose, J C
AU  - Rose JC
LA  - eng
GR  - R01 HD017644/HD/NICHD NIH HHS/United States
GR  - HD-1120/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
SB  - IM
MH  - Adrenocorticotropic Hormone/*blood
MH  - Animals
MH  - Biological Assay
MH  - Female
MH  - Fetal Diseases/*blood
MH  - Gestational Age
MH  - Hemorrhage/blood
MH  - Pregnancy
MH  - Radioimmunoassay
MH  - Sheep/*blood
MH  - Stress, Physiological/*blood/physiopathology
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - 10.1152/ajpendo.1993.265.1.E68 [doi]
PST - ppublish
SO  - Am J Physiol. 1993 Jul;265(1 Pt 1):E68-73. doi: 10.1152/ajpendo.1993.265.1.E68.