PMID- 8393415
OWN - NLM
STAT- MEDLINE
DCOM- 19930902
LR  - 20190512
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 109
IP  - 2-3
DP  - 1993 May 15
TI  - Large scale production and purification of human retrovirus-like particles 
      related to the mouse mammary tumor virus.
PG  - 289-96
AB  - Human retrovirus-like particles related to mouse mammary tumor virus (MMTV) are 
      secreted in a steroid-dependent manner by the breast cancer cell line T47D. We 
      report the successful large scale production and purification of these particles 
      from culture supernatants of T47D cells and describe the experimental conditions 
      established for this purpose. Thus, mg amounts of particles were produced by 
      large scale culturing of T47D cells in an autoharvesting roller bottle system and 
      purified by differential centrifugation and continuous flow ultracentrifugation 
      on density gradients with a 50% recovery and a 350-fold enrichment.
FAU - Faff, O
AU  - Faff O
AD  - Medizinische Poliklinik, Universitat Munchen, FRG.
FAU - Murray, B A
AU  - Murray BA
FAU - Erfle, V
AU  - Erfle V
FAU - Hehlmann, R
AU  - Hehlmann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
SB  - IM
MH  - Animals
MH  - Breast Neoplasms
MH  - Cell Division
MH  - Humans
MH  - Mammary Tumor Virus, Mouse/growth & development/isolation & purification
MH  - Microscopy, Electron
MH  - Retroviridae/*growth & development/isolation & purification
MH  - Tumor Cells, Cultured
MH  - *Virus Cultivation
EDAT- 1993/05/15 00:00
MHDA- 1993/05/15 00:01
CRDT- 1993/05/15 00:00
PHST- 1993/05/15 00:00 [pubmed]
PHST- 1993/05/15 00:01 [medline]
PHST- 1993/05/15 00:00 [entrez]
AID - 10.1016/0378-1097(93)90035-z [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 1993 May 15;109(2-3):289-96. doi: 
      10.1016/0378-1097(93)90035-z.