PMID- 8394003
OWN - NLM
STAT- MEDLINE
DCOM- 19930907
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 90
IP  - 15
DP  - 1993 Aug 1
TI  - Reconstitution of retinoid X receptor function and combinatorial regulation of 
      other nuclear hormone receptors in the yeast Saccharomyces cerevisiae.
PG  - 6929-33
AB  - The nuclear hormone receptor family of transcription factors regulates gene 
      expression via a complex combinatorial network of interactions. Of particular 
      interest is the ability of retinoid X receptors (RXRs) to form heterodimers with 
      retinoic acid receptors (RARs) and thyroid hormone receptors (TRs), thereby 
      modifying their activities. We report here that RXR, RAR, and TR function can be 
      reconstituted in the yeast Saccharomyces cerevisiae and demonstrate that the 
      combinatorial regulation seen in vertebrate cells can be reproduced in the yeast 
      background. Using this system, we have shown that RARs respond to a wide variety 
      of retinoid ligands but that RXRs are specific for the 9-cis isomer of retinoic 
      acid. RXR enhanced the activity of RARs and TRs on a variety of hormone response 
      elements without demonstrably altering their DNA specificity. Interestingly, the 
      ability of RXR to potentiate gene activation by RARs and by TRs varied for 
      different receptor isoforms.
FAU - Hall, B L
AU  - Hall BL
AD  - Department of Microbiology, University of California, Davis 95616.
FAU - Smit-McBride, Z
AU  - Smit-McBride Z
FAU - Privalsky, M L
AU  - Privalsky ML
LA  - eng
GR  - CA53394/CA/NCI NIH HHS/United States
GR  - F32 CA09050/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Receptors, Thyroid Hormone)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Base Sequence
MH  - Binding Sites
MH  - Carrier Proteins/*physiology
MH  - DNA-Binding Proteins/metabolism
MH  - *Gene Expression Regulation
MH  - In Vitro Techniques
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Receptors, Cell Surface/*physiology
MH  - Receptors, Retinoic Acid
MH  - Receptors, Thyroid Hormone/*physiology
MH  - Regulatory Sequences, Nucleic Acid
MH  - Retinoid X Receptors
MH  - Saccharomyces cerevisiae
MH  - *Transcription Factors
MH  - Transcription, Genetic
MH  - Transcriptional Activation
PMC - PMC47048
EDAT- 1993/08/01 00:00
MHDA- 1993/08/01 00:01
PMCR- 1994/02/01
CRDT- 1993/08/01 00:00
PHST- 1993/08/01 00:00 [pubmed]
PHST- 1993/08/01 00:01 [medline]
PHST- 1993/08/01 00:00 [entrez]
PHST- 1994/02/01 00:00 [pmc-release]
AID - 10.1073/pnas.90.15.6929 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):6929-33. doi: 
      10.1073/pnas.90.15.6929.