PMID- 8395841
OWN - NLM
STAT- MEDLINE
DCOM- 19930928
LR  - 20181130
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 195
IP  - 1
DP  - 1993 Aug 31
TI  - Modulation of beta-amyloid precursor protein secretion in differentiated and 
      nondifferentiated cells.
PG  - 97-103
AB  - The regulation of beta-amyloid precursor protein (beta-APP) secretion was 
      compared in differentiated and nondifferentiated PC12 and B104 cells. Phorbol 
      esters stimulated the release of beta-APP in all cells examined. However, 
      differentiated PC12 cells were much more sensitive to phorbol ester treatment 
      than nondifferentiated PC12 cells and their beta-APP release was also induced by 
      the protein phosphatase inhibitor okadaic acid and the Ca(++)-ionophore A23187. 
      In contrast, beta-APP release from B104 cells was strongly stimulated by A23187 
      and to lesser degree by phorbol esters. This effect was most pronounced in 
      nondifferentiated B104 cells, which might be due to a higher basic release of 
      beta-APP from differentiated B104 cells. Thus, the regulation of beta-APP 
      cleavage and release varies depending on the cell type and differentiation state 
      of the cell.
FAU - Loffler, J
AU  - Loffler J
AD  - Pharma Division, F. Hoffmann-La Roche Ltd, Basel, Switzerland.
FAU - Huber, G
AU  - Huber G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Carcinogens)
RN  - 0 (Culture Media)
RN  - 0 (Phorbol Esters)
RN  - 24928-17-4 (phorbol-12,13-didecanoate)
RN  - 37558-16-0 (Phorbol 12,13-Dibutyrate)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Amyloid beta-Protein Precursor/*biosynthesis/isolation & purification/metabolism
MH  - Animals
MH  - Bucladesine/*pharmacology
MH  - Calcimycin/pharmacology
MH  - Carcinogens/pharmacology
MH  - *Cell Differentiation/drug effects
MH  - Cell Line
MH  - Culture Media
MH  - PC12 Cells
MH  - Phorbol 12,13-Dibutyrate/pharmacology
MH  - Phorbol Esters/*pharmacology
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1993/08/31 00:00
MHDA- 1993/08/31 00:01
CRDT- 1993/08/31 00:00
PHST- 1993/08/31 00:00 [pubmed]
PHST- 1993/08/31 00:01 [medline]
PHST- 1993/08/31 00:00 [entrez]
AID - S0006-291X(83)72015-2 [pii]
AID - 10.1006/bbrc.1993.2015 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1993 Aug 31;195(1):97-103. doi: 
      10.1006/bbrc.1993.2015.