PMID- 8396052
OWN - NLM
STAT- MEDLINE
DCOM- 19931005
LR  - 20190830
IS  - 0020-711X (Print)
IS  - 0020-711X (Linking)
VI  - 25
IP  - 7
DP  - 1993 Jul
TI  - Effect of insulin and glucagon on the mobility of ESR-probes incorporated in rat 
      liver plasma membranes.
PG  - 971-7
AB  - 1. The effect of different concentrations of insulin (INS) and glucagon (GLU) on 
      the rotational mobility of a membrane-incorporated spin probe 
      2,2-6,6-tetramethyl-4-capriloyl piperidine-1-oxil (C7) was investigated by 
      electron spin resonance (ESR) technique. 2. Two strong adenylate cyclase 
      effectors guanosine 5'-triphosphate (GTP) and guanylyl 5'-imidodiphosphate 
      [Gpp(NH)p], as well as an antioxidant 4-methyl-2,6-ditretbutilphenol (AO) and a 
      nonprotein hormone prostaglandin E2 (PGE2) were used as reference effectors. 3. 
      Applied effectors reduced by 30-82% the rotation correlation time (TR) of the rat 
      liver plasma membranes spin probe C7. The effect was time-dependent and reached 
      saturation 30-40 min after the effector application. 4. The kinetic- and 
      concentration-dependent changes in TR were described by a simple phenomenological 
      model. The apparent binding constants and the number of the apparent membrane 
      binding sites of the effectors used were calculated using the model.
FAU - Zlatanov, I
AU  - Zlatanov I
AD  - Central Lab of Biophysics, Bulgarian Academy of Sciences, Sofia.
FAU - Maltzeva, E
AU  - Maltzeva E
FAU - Borovok, N
AU  - Borovok N
FAU - Spassov, V
AU  - Spassov V
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Biochem
JT  - The International journal of biochemistry
JID - 0250365
RN  - 0 (Caprylates)
RN  - 0 (Cyclic N-Oxides)
RN  - 0 (Insulin)
RN  - 0 (Spin Labels)
RN  - 126328-27-6 (2,2,6,6-tetramethyl-4-capryloylpiperidine-1-oxyl)
RN  - 1P9D0Z171K (Butylated Hydroxytoluene)
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 9007-92-5 (Glucagon)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Adenylyl Cyclases/metabolism
MH  - Animals
MH  - Butylated Hydroxytoluene/pharmacology
MH  - Caprylates
MH  - Cell Membrane/metabolism
MH  - Cyclic N-Oxides
MH  - Dinoprostone/pharmacology
MH  - Electron Spin Resonance Spectroscopy
MH  - Enzyme Activation
MH  - Glucagon/*pharmacology
MH  - Guanosine Triphosphate/pharmacology
MH  - Guanylyl Imidodiphosphate/pharmacology
MH  - Insulin/*pharmacology
MH  - Kinetics
MH  - Liver/cytology/*metabolism
MH  - Membrane Fluidity/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - *Spin Labels
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - 10.1016/0020-711x(93)90109-r [doi]
PST - ppublish
SO  - Int J Biochem. 1993 Jul;25(7):971-7. doi: 10.1016/0020-711x(93)90109-r.