PMID- 8396389
OWN - NLM
STAT- MEDLINE
DCOM- 19931005
LR  - 20190904
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 67
IP  - 5
DP  - 1993
TI  - Effects of cisplatin and ORG.2766 in chick embryonic brain cell cultures.
PG  - 325-9
AB  - The neurotoxicity of cisplatin and ORG.2766, both separately and in combination, 
      was investigated using serum-free chick embryonic brain cell cultures. At low 
      cisplatin concentrations glial cells were affected more than nerve cells. The 
      onset of cisplatin toxicity was delayed, the major effects only being seen after 
      the treatment was finished and when no free cisplatin was present in the culture 
      medium. The data further indicate that the area under the graph of free cisplatin 
      concentration in the culture medium against time (AUC) is a measure of cisplatin 
      toxicity. The AUC of free cisplatin in the culture medium which causes a 50% 
      reduction in the expression of glial fibrillary associated protein (GFAP) was 
      similar to blood AUC values in humans known to induce neurotoxic effects in 
      around 80% of patients. ORG.2766 at very high concentrations increased lysosomal 
      activity, as measured by neutral red uptake, and the expression of GFAP. ORG.2766 
      did not influence the toxicity of cisplatin in culture.
FAU - Bruinink, A
AU  - Bruinink A
AD  - Institute of Toxicology, Swiss Federal Institute of Technology, Schwerzenbach.
FAU - Birchler, F
AU  - Birchler F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Peptide Fragments)
RN  - 50913-82-1 (Org 2766)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Adrenocorticotropic Hormone/*analogs & derivatives/pharmacology
MH  - Animals
MH  - Antineoplastic Agents/*pharmacology
MH  - Brain/*cytology/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Cisplatin/*antagonists & inhibitors/pharmacokinetics/toxicity
MH  - Glial Fibrillary Acidic Protein/biosynthesis
MH  - Half-Life
MH  - Lysosomes/drug effects
MH  - Neuroglia/drug effects
MH  - Neurons/drug effects
MH  - Peptide Fragments/*pharmacology
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1007/BF01973703 [doi]
PST - ppublish
SO  - Arch Toxicol. 1993;67(5):325-9. doi: 10.1007/BF01973703.