PMID- 8397102 OWN - NLM STAT- MEDLINE DCOM- 19931018 LR - 20190621 IS - 0014-5793 (Print) IS - 0014-5793 (Linking) VI - 330 IP - 3 DP - 1993 Sep 20 TI - Expression of low-affinity NGF receptor and trkB mRNA in human SH-SY5Y neuroblastoma cells. PG - 287-92 AB - We have used the human neuroblastoma cell line SH-SY5Y as a model system to investigate the expression and regulation of the receptors for brain-derived neurotrophic factor (BDNF), a member of the nerve growth factor (NGF) family of neurotrophins. We demonstrate that SH-SY5Y cells express transcripts encoding the low-affinity NGF receptor (LNGFR) and trkB, the signal transducing receptor unit for BDNF. Interaction of BDNF with SH-SY5Y cells increased the transcription of the c-fos gene, showing that these molecules encode functional BDNF receptors. Our findings that differentiating agents such as retinoids and cAMP analogs increased the expression of LNGFR, but decreased trkB mRNA levels, suggest that LNGFR and trkB have different roles during neuronal differentiation. FAU - Ehrhard, P B AU - Ehrhard PB AD - Department of Physiology, University of Basel, Vesalianum, Switzerland. FAU - Ganter, U AU - Ganter U FAU - Schmutz, B AU - Schmutz B FAU - Bauer, J AU - Bauer J FAU - Otten, U AU - Otten U LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - FEBS Lett JT - FEBS letters JID - 0155157 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (DNA, Single-Stranded) RN - 0 (Membrane Proteins) RN - 0 (Nerve Tissue Proteins) RN - 0 (Proto-Oncogene Proteins c-fos) RN - 0 (RNA, Messenger) RN - 0 (Receptor, Ciliary Neurotrophic Factor) RN - 0 (Receptors, Nerve Growth Factor) RN - 0 (Retinoids) RN - 0 (brain-derived growth factor) RN - 63X7MBT2LQ (Bucladesine) RN - EC 2.7.10.1 (Protein-Tyrosine Kinases) SB - IM GS - c-fos MH - Base Sequence MH - *Brain-Derived Neurotrophic Factor MH - Bucladesine/pharmacology MH - DNA, Single-Stranded MH - Gene Expression/drug effects MH - Humans MH - Membrane Proteins/*genetics MH - Molecular Sequence Data MH - Nerve Tissue Proteins/metabolism MH - Neuroblastoma MH - Protein-Tyrosine Kinases/*genetics MH - Proto-Oncogene Proteins c-fos/biosynthesis/genetics MH - RNA, Messenger/drug effects/genetics/*metabolism MH - Receptor, Ciliary Neurotrophic Factor MH - Receptors, Nerve Growth Factor/biosynthesis/*genetics/metabolism MH - Retinoids/pharmacology MH - Tumor Cells, Cultured EDAT- 1993/09/20 00:00 MHDA- 1993/09/20 00:01 CRDT- 1993/09/20 00:00 PHST- 1993/09/20 00:00 [pubmed] PHST- 1993/09/20 00:01 [medline] PHST- 1993/09/20 00:00 [entrez] AID - 0014-5793(93)80890-7 [pii] AID - 10.1016/0014-5793(93)80890-7 [doi] PST - ppublish SO - FEBS Lett. 1993 Sep 20;330(3):287-92. doi: 10.1016/0014-5793(93)80890-7.