PMID- 8397282
OWN - NLM
STAT- MEDLINE
DCOM- 19931018
LR  - 20090929
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 74 ( Pt 9)
DP  - 1993 Sep
TI  - Identification and expression of the human herpesvirus 6 glycoprotein H and 
      interaction with an accessory 40K glycoprotein.
PG  - 1847-57
AB  - In herpes simplex virus (HSV) the small secreted glycoprotein gL forms a 
      heterodimer with the transmembrane envelope glycoprotein gH. Here we identify the 
      human herpesvirus 6 (HHV-6) gL gene, express HHV-6 gL and gH homologues, and 
      examine interactions between HHV-6 gH and gL. The HHV-6 gL gene encoded a 
      glycoprotein with an amino acid sequence which showed closest similarity to the 
      human cytomegalovirus (HCMV) gL homologue (18% identity). Products of HHV-6 gH 
      and gL genes were characterized in an in vitro transcription-translation system 
      and in a transient in vivo expression system. Both gH and gL were transcribed and 
      translated in vitro to give products of apparent M(r) of 65K and 28K in SDS-PAGE, 
      and these could be processed by addition of microsomes to 110K and 40K, 
      respectively. To study gH/gL interactions, gH was tagged with the nine amino acid 
      epitope for monoclonal antibody LP14 (anti-HSV-1 gD). LP14 and a human serum 
      sample specifically immunoprecipitated gH and a stable complex of gH and gL 
      co-expressed in an in vivo vaccinia virus-T7 system. The gH and gL produced in 
      this in vivo expression system corresponded to the M(r)s of the fully processed 
      glycoproteins identified in the in vitro system. The gH expressed together with 
      gL was recognized by human sera more easily than when examined on its own in 
      immunofluorescence assays. Dual expression of gH and gL in transfected T 
      lymphocytes (JJhan) caused reactions with 75% of human sera tested (12 
      HHV-6-positive, HCMV-negative serum samples), but gL expressed alone was not 
      recognized by these sera. The immunofluorescence studies also showed that the 
      glycoproteins were localized in Golgi-like bodies in fibroblasts, but occurred 
      throughout the endoplasmic reticulum in T lymphocytes, the normal cellular target 
      for HHV-6. These results show the identification of the HHV-6 homologue to the 
      HCMV and HSV gL genes, identification and production of HHV-6 gH and gL expressed 
      both in vitro and in vivo, complex formation between these glycoproteins, and 
      evidence that this complex may be localized differently in fibroblasts as 
      compared to T lymphocytes and that it is immunogenic.
FAU - Liu, D X
AU  - Liu DX
AD  - Department of Medicine, University of Cambridge, Addenbrooke's Hospital, U.K.
FAU - Gompels, U A
AU  - Gompels UA
FAU - Nicholas, J
AU  - Nicholas J
FAU - Lelliott, C
AU  - Lelliott C
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 142985-75-9 (glycoprotein H, herpesvirus 6)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Bone Marrow Transplantation
MH  - Cell Line
MH  - Cytomegalovirus/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fluorescent Antibody Technique
MH  - Gene Expression
MH  - Herpesvirus 6, Human/*genetics/isolation & purification/*metabolism
MH  - Humans
MH  - Microsomes/metabolism
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Mutagenesis, Site-Directed
MH  - Oligodeoxyribonucleotides
MH  - Polymerase Chain Reaction
MH  - Protein Biosynthesis
MH  - RNA, Messenger/metabolism
MH  - Recombinant Proteins/biosynthesis/isolation & purification/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
MH  - Transfection
MH  - Viral Envelope Proteins/biosynthesis/isolation & purification/*metabolism
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1099/0022-1317-74-9-1847 [doi]
PST - ppublish
SO  - J Gen Virol. 1993 Sep;74 ( Pt 9):1847-57. doi: 10.1099/0022-1317-74-9-1847.