PMID- 8397410
OWN - NLM
STAT- MEDLINE
DCOM- 19931020
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 90
IP  - 18
DP  - 1993 Sep 15
TI  - In vitro analysis of Ah receptor domains involved in ligand-activated DNA 
      recognition.
PG  - 8566-70
AB  - The Ah receptor (AHR) is a basic helix-loop-helix protein that mediates the 
      effects of 2,3,7,8-tetrachloro-dibenzo-p-dioxin. In this report, we describe a 
      rabbit reticulocyte system that allows functional expression of both the AHR and 
      its dimeric partner, the AHR nuclear translocator protein (ARNT). By using this 
      in vitro system, we were able to reconstitute agonist binding to the AHR and 
      agonist-induced AHR-ARNT recognition of a cognate DNA enhancer sequence. 
      Expression of AHR deletion mutants revealed the location of N-terminal domains 
      responsible for ligand and DNA recognition and C-terminal domains that play roles 
      in agonist-induced DNA recognition.
FAU - Dolwick, K M
AU  - Dolwick KM
AD  - Department of Pharmacology, Northwestern University Medical School, Chicago, IL 
      60611.
FAU - Swanson, H I
AU  - Swanson HI
FAU - Bradfield, C A
AU  - Bradfield CA
LA  - eng
SI  - GENBANK/M94623
GR  - ES-05589/ES/NIEHS NIH HHS/United States
GR  - ES-05703/ES/NIEHS NIH HHS/United States
GR  - T32-CA09560/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Codon)
RN  - 0 (Ligands)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Receptors, Drug)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Blotting, Western
MH  - Codon
MH  - DNA/*metabolism
MH  - Ligands
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Oligodeoxyribonucleotides
MH  - Polychlorinated Dibenzodioxins/metabolism
MH  - Polymerase Chain Reaction
MH  - Protein Biosynthesis
MH  - Rabbits
MH  - Receptors, Aryl Hydrocarbon
MH  - Receptors, Drug/genetics/*metabolism
MH  - Reticulocytes/metabolism
MH  - Sequence Deletion
MH  - Transcription, Genetic
PMC - PMC47398
EDAT- 1993/09/15 00:00
MHDA- 1993/09/15 00:01
PMCR- 1994/03/15
CRDT- 1993/09/15 00:00
PHST- 1993/09/15 00:00 [pubmed]
PHST- 1993/09/15 00:01 [medline]
PHST- 1993/09/15 00:00 [entrez]
PHST- 1994/03/15 00:00 [pmc-release]
AID - 10.1073/pnas.90.18.8566 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8566-70. doi: 
      10.1073/pnas.90.18.8566.