PMID- 8400289
OWN - NLM
STAT- MEDLINE
DCOM- 19931123
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 82
IP  - 8
DP  - 1993 Oct 15
TI  - Both megakaryocytopoiesis and erythropoiesis are induced in mice infected with a 
      retrovirus expressing an oncogenic erythropoietin receptor.
PG  - 2386-95
AB  - Increasing direct and indirect evidence suggests that erythropoietin (Epo) 
      promotes both erythropoiesis and megakaryocytopoiesis. Here we report that, in 
      mice infected with a recombinant spleen focus-forming retrovirus (SFFV) 
      expressing an oncogenic erythropoietin receptor (EpoR), there was an increase in 
      platelet count preceding the ensuing erythrocytosis. Concurrently, there was a 
      substantial increase in splenic megakaryocytes. Culture of the bone marrow and 
      spleen cells from infected mice showed enhanced numbers of multipotent 
      megakaryocytic progenitors. DNA polymerase chain reaction analysis of individual 
      megakaryocyte-containing colonies showed recombinant SFFV (SFFVcEpoR) proviral 
      integration. Immunofluorescence of spleen sections showed overexpression of EpoR 
      protein in the megakaryocytes. Mice infected with a strain of SFFV also developed 
      splenic megakaryocytosis without activating overexpression of the EpoR in 
      megakaryocytes. This in vivo system shows that a relationship between 
      erythropoiesis and thrombopoiesis can exist at the level of the Epo-EpoR 
      signaling pathway. Also, SFFV-based vectors may be excellent vehicles for the 
      introduction of genes into multipotent, hematopoietic progenitors, in vitro.
FAU - Longmore, G D
AU  - Longmore GD
AD  - Department of Medicine, Washington University School of Medicine, St Louis, MO 
      63110.
FAU - Pharr, P
AU  - Pharr P
FAU - Neumann, D
AU  - Neumann D
FAU - Lodish, H F
AU  - Lodish HF
LA  - eng
GR  - HL32262/HL/NHLBI NIH HHS/United States
GR  - NIA AG00294/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Receptors, Erythropoietin)
SB  - IM
CIN - Blood. 1994 Jun 15;83(12):3829-31. PMID: 8204902
MH  - Animals
MH  - Base Sequence
MH  - *Erythropoiesis
MH  - Female
MH  - *Hematopoiesis
MH  - Leukemia, Experimental/physiopathology
MH  - Megakaryocytes/*physiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Receptors, Erythropoietin/analysis/biosynthesis/*physiology
MH  - Retroviridae Infections/*physiopathology
MH  - Spleen Focus-Forming Viruses/genetics
MH  - Tumor Virus Infections/physiopathology
EDAT- 1993/10/15 00:00
MHDA- 1993/10/15 00:01
CRDT- 1993/10/15 00:00
PHST- 1993/10/15 00:00 [pubmed]
PHST- 1993/10/15 00:01 [medline]
PHST- 1993/10/15 00:00 [entrez]
AID - S0006-4971(20)84032-4 [pii]
PST - ppublish
SO  - Blood. 1993 Oct 15;82(8):2386-95.