PMID- 8400640
OWN - NLM
STAT- MEDLINE
DCOM- 19931123
LR  - 20191023
IS  - 0890-6238 (Print)
IS  - 0890-6238 (Linking)
VI  - 7 Suppl 1
DP  - 1993
TI  - Detection of genetic damage in human sperm.
PG  - 47-52
AB  - Assessment of genetic damage in human sperm is now possible using the hamster 
      oocyte/human sperm fusion technique. This cross-species in vitro assay provides 
      precise human sperm karyotypes that can be banded for identification of 
      individual chromosomes and numeric and structural abnormalities. This technique 
      is time-consuming and difficult but there are now 11 laboratories in 7 different 
      countries reporting results on normal men and men at increased risk of sperm 
      chromosomal abnormalities. Men who have been exposed to radiotherapy and 
      chemotherapy have been shown to have an increased frequency of chromosomal 
      abnormalities in their sperm. A few laboratories have reported results on the use 
      of fluorescence in situ hybridization (FISH) of interphase sperm nuclei. This 
      appears to be a promising new technique for the assessment of aneuploidy and FISH 
      results from our laboratory correlate well with our frequency of aneuploidy 
      determined by sperm chromosome analysis. Thus it is possible that, in the future, 
      FISH might be used as a simple rapid screen of potentially aneugenic agents.
FAU - Martin, R H
AU  - Martin RH
AD  - Department of Pediatrics, Faculty of Medicine, University of Calgary, Alberta, 
      Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
SB  - IM
MH  - Animals
MH  - *Chromosome Aberrations
MH  - Cricetinae
MH  - Drug Evaluation, Preclinical/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Spermatozoa/*ultrastructure
RF  - 38
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1016/0890-6238(93)90068-i [doi]
PST - ppublish
SO  - Reprod Toxicol. 1993;7 Suppl 1:47-52. doi: 10.1016/0890-6238(93)90068-i.