PMID- 8402448
OWN - NLM
STAT- MEDLINE
DCOM- 19931112
LR  - 20190620
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 72
IP  - 8
DP  - 1993 Oct 15
TI  - Characterization of lung squamous cell carcinoma-derived T-cell suppressive 
      factor.
PG  - 2347-57
AB  - BACKGROUND: The immunosuppressive state of a tumor-bearing patient is possibly 
      mediated by tumor-derived factor. In this study, the authors characterized lung 
      squamous cell carcinoma-derived immunosuppressive factor (LSCF). METHODS: The 
      immunosuppressive activity of QG56 (a lung squamous carcinoma cell line)-derived 
      LSCF was evaluated by the effect of culture supernatant of QG56 on anti-CD3 
      monoclonal antibody-induced T-cell, response such as proliferation (3H-thymidine 
      uptake), cytotoxicity (51Cr-releasing assay), and expression of cytokine mRNA 
      (polymerase chain reaction). The LSCF was partially purified with an ion-exchange 
      high-performance liquid chromatography (HPLC) and a gel-filtration HPLC. RESULTS: 
      The LSCF inhibited proliferation, cytotoxicity, and expression of cytokine mRNA 
      of T-cells in a dose-dependent manner. It has a molecular weight of approximately 
      22 kd, and was sensitive to proteinase K, heating at 60 degrees C, and resistant 
      to treatment with trypsin and pH 3 and 9. These properties appear to be similar 
      to those of transforming growth factor-beta (TGF-beta). However, the activity of 
      the LSCF was not abrogated by anti-TGF-beta sera, and the LSCF did not suppress 
      the proliferation of TGF-beta-sensitive mink lung cells (Mv1Lu). CONCLUSIONS: 
      These data suggest that LSCF may be a novel tumor-derived immunosuppressive 
      protein factor.
FAU - Yoshino, I
AU  - Yoshino I
AD  - Department of Virology, Kyushu University, Fukuoka, Japan.
FAU - Yano, T
AU  - Yano T
FAU - Miyamoto, M
AU  - Miyamoto M
FAU - Yamada, K
AU  - Yamada K
FAU - Kajii, Y
AU  - Kajii Y
FAU - Onodera, K
AU  - Onodera K
FAU - Ishida, T
AU  - Ishida T
FAU - Sugimachi, K
AU  - Sugimachi K
FAU - Kimura, G
AU  - Kimura G
FAU - Nomoto, K
AU  - Nomoto K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (CD3 Complex)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin-2)
RN  - 0 (Suppressor Factors, Immunologic)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Base Sequence
MH  - CD3 Complex/immunology
MH  - Carcinoma, Squamous Cell/*immunology/metabolism
MH  - Cytokines/genetics
MH  - Cytotoxicity, Immunologic
MH  - DNA Primers
MH  - Humans
MH  - Interleukin-2/metabolism
MH  - Lung Neoplasms/*immunology/metabolism
MH  - Lymphocyte Activation
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - RNA, Messenger/metabolism
MH  - Receptors, Interleukin-2/metabolism
MH  - Suppressor Factors, Immunologic/metabolism/*physiology
MH  - T-Lymphocytes/*immunology/metabolism
MH  - Transforming Growth Factor beta/physiology
MH  - Tumor Cells, Cultured
EDAT- 1993/10/15 00:00
MHDA- 1993/10/15 00:01
CRDT- 1993/10/15 00:00
PHST- 1993/10/15 00:00 [pubmed]
PHST- 1993/10/15 00:01 [medline]
PHST- 1993/10/15 00:00 [entrez]
AID - 10.1002/1097-0142(19931015)72:8<2347::aid-cncr2820720810>3.0.co;2-i [doi]
PST - ppublish
SO  - Cancer. 1993 Oct 15;72(8):2347-57. doi: 
      10.1002/1097-0142(19931015)72:8<2347::aid-cncr2820720810>3.0.co;2-i.