PMID- 8402942
OWN - NLM
STAT- MEDLINE
DCOM- 19931105
LR  - 20131121
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 151
IP  - 2
DP  - 1993 Oct 15
TI  - Impaired bone marrow-derived macrophage differentiation in vitamin D deficiency.
PG  - 356-68
AB  - The calcium-regulating hormone, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), is also 
      recognized as an immunomodulator. In vitro addition of the hormone to bone 
      marrow-derived macrophages (BMDMs) results in a decreased proliferation and an 
      increased differentiation. In the present work we compare the in vitro 
      differentiation of BMDMs derived from vitamin D-depleted and -repleted mice (-D 
      and +D BMDMs, respectively). -D BMDMs proliferate in vitro slower than +D BMDMs. 
      Addition of the hormone to BMDM cultures inhibited the rate of their 
      proliferation, which was more pronounced in low-density cultures. The ability of 
      mononuclear phagocytes to produce reactive oxygen metabolites is an important 
      element in the microbicidal functions of these cells. We found that -D BMDMs 
      produce less H2O2 than +D BMDMs, which was corrected by the in vitro addition of 
      1,25(OH)2D3. Analyses of various macrophage-specific surface antigens revealed a 
      reduction in their expression on -D BMDMs. In vitro addition of 1,25(OH)2D3 to 
      BMDM cultures increased the expression of these antigens. The activity of the 
      lysosomal enzyme acid phosphatase was similarly affected by vitamin D deficiency 
      and by the in vitro addition of the hormone. Thus, vitamin D deficiency is 
      associated with impaired maturation of BMDMs suggesting that the hormone is a 
      natural modulator of macrophage maturation.
FAU - Abu-Amer, Y
AU  - Abu-Amer Y
AD  - Hubert Humphrey Center for Experimental Medicine and Cancer Research, Hebrew 
      University-Hadassah Medical School, Jerusalem, Israel.
FAU - Bar-Shavit, Z
AU  - Bar-Shavit Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antigens, Surface)
RN  - 1C6V77QF41 (Cholecalciferol)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/biosynthesis
MH  - *Bone Marrow Cells
MH  - Cell Differentiation/physiology
MH  - Cell Division/physiology
MH  - Cells, Cultured
MH  - Cholecalciferol/*physiology
MH  - Hydrogen Peroxide/metabolism
MH  - Lysosomes/enzymology
MH  - Macrophages/*cytology/physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Vitamin D Deficiency/immunology
EDAT- 1993/10/15 00:00
MHDA- 1993/10/15 00:01
CRDT- 1993/10/15 00:00
PHST- 1993/10/15 00:00 [pubmed]
PHST- 1993/10/15 00:01 [medline]
PHST- 1993/10/15 00:00 [entrez]
AID - S0008-8749(83)71245-1 [pii]
AID - 10.1006/cimm.1993.1245 [doi]
PST - ppublish
SO  - Cell Immunol. 1993 Oct 15;151(2):356-68. doi: 10.1006/cimm.1993.1245.