PMID- 8406447
OWN - NLM
STAT- MEDLINE
DCOM- 19931022
LR  - 20071114
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 17
IP  - 1
DP  - 1993 Jul
TI  - High-resolution mapping of probes near the X-linked lymphoproliferative disease 
      (XLP) locus.
PG  - 163-70
AB  - Fluorescence in situ hybridization (FISH) was employed in high-resolution mapping 
      of probes near the X-linked lymphoproliferative disease (XLP) locus. The map 
      includes the DXS42, DXS12, DXS6, DXS982, DXS739, DXS75, DXS100, DXS10, and DXS177 
      loci. Metaphase analysis showed that DXS12 and DXS42 mapped to proximal Xq25, 
      while DXS10 and DXS177 mapped to proximal Xq26.1. DXS6, DXS982, DXS739, DXS75, 
      and DXS100 were in Xq25. The order of probes deduced from interphase FISH was: 
      Xq24-(DXS12, DXS42)-DXS6-DXS982-DXS739-DXS75-DXS100+ ++-DXS10-DXS177-Xq26.2. We 
      estimate that the entire region between DXS12 and DXS177 is about 7 Mb. Our 
      previous study indicated that all three XLP deletions (63-3, 66-1, and 43-4) 
      lacked DXS739. We now report that DXS75 and DXS982 are also missing in these 
      deletions. Using interphase FISH measurements, we estimate that 2 Mb are absent 
      in 63-3, and 4 Mb are absent in 66-1 and 43-4. This FISH map confines the XLP 
      candidate gene region to a 2-Mb interval between DXS6 and DXS100 and places 
      DXS100 distal to the XLP locus. This study also demonstrates that small probes 
      (0.6 to 3.6 kb) can be utilized in FISH.
FAU - Wu, B L
AU  - Wu BL
AD  - Center for Human Genetics, Boston University School of Medicine, Massachusetts 
      02118-2394.
FAU - Milunsky, A
AU  - Milunsky A
FAU - Nelson, D
AU  - Nelson D
FAU - Schmeckpeper, B
AU  - Schmeckpeper B
FAU - Porta, G
AU  - Porta G
FAU - Schlessinger, D
AU  - Schlessinger D
FAU - Skare, J
AU  - Skare J
LA  - eng
GR  - CA46929/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
SB  - IM
GS  - LYP
MH  - *Chromosome Deletion
MH  - *DNA Probes
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Lymphoproliferative Disorders/*genetics
MH  - Male
MH  - Metaphase
MH  - Sex Chromosome Aberrations/*genetics
MH  - *X Chromosome
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - S0888-7543(83)71298-X [pii]
AID - 10.1006/geno.1993.1298 [doi]
PST - ppublish
SO  - Genomics. 1993 Jul;17(1):163-70. doi: 10.1006/geno.1993.1298.