PMID- 8406476
OWN - NLM
STAT- MEDLINE
DCOM- 19931022
LR  - 20061115
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 17
IP  - 1
DP  - 1993 Jul
TI  - A fluorescence in situ hybridization map of human chromosome 21 consisting of 30 
      genetic and physical markers on the chromosome: localization of 137 additional 
      YAC and cosmid clones with respect to this map.
PG  - 98-105
AB  - A fluorescence in situ hybridization (FISH) map of human chromosome 21 was 
      compiled using yeast artificial chromosome (YAC) DNA probes that encode 28 
      markers physically and/or genetically mapped on the chromosome. Probes that 
      recognize the centromere and rDNA repeat sequences in the p arm were also placed 
      as reference markers on the FISH map. For each probe, the location of the 
      fluorescence hybridization signal was measured on metaphase chromosomes with 
      respect to fractional chromosome length (FL) from p-ter. The location of the 
      markers was established with a standard error of +/- 1.9 Mb using from 9 to 63 FL 
      measurements for each probe. The relative order and separation of the markers as 
      determined by FISH are shown to correspond well to those of other maps of the 
      chromosome. Fifty-one additional YAC and 86 cosmid clones were also localized by 
      FISH with respect to the 30 markers on the chromosome. The cosmids, chosen at 
      random from a flow-sorter chromosome 21 cosmid library, show some biases in 
      chromosome distribution.
FAU - Gingrich, J C
AU  - Gingrich JC
AD  - Human Genome Center, Lawrence Berkeley Laboratory, California 94720.
FAU - Shadravan, F
AU  - Shadravan F
FAU - Lowry, S R
AU  - Lowry SR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Fungal
MH  - *Chromosomes, Human, Pair 21
MH  - Cosmids
MH  - DNA Probes
MH  - Gene Library
MH  - Genetic Markers
MH  - Genome, Human
MH  - Humans
MH  - Hybrid Cells
MH  - *In Situ Hybridization, Fluorescence
MH  - Sensitivity and Specificity
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
AID - S0888-7543(83)71289-9 [pii]
AID - 10.1006/geno.1993.1289 [doi]
PST - ppublish
SO  - Genomics. 1993 Jul;17(1):98-105. doi: 10.1006/geno.1993.1289.