PMID- 8406994
OWN - NLM
STAT- MEDLINE
DCOM- 19931122
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 55
IP  - 4
DP  - 1993 Oct 21
TI  - Induction and release of manganese superoxide dismutase from mitochondria of 
      human umbilical vein endothelial cells by tumor necrosis factor-alpha and 
      interleukin-1 alpha.
PG  - 646-50
AB  - The effects of tumor necrosis factor-alpha (TNF alpha) on cultured human 
      umbilical vein endothelial cells (EC) and 2 cancer cell lines, A549 and ME180, 
      were compared. The effects of interleukin-1 alpha (IL-1 alpha) on EC were also 
      examined. While A549 cells were fairly resistant to the cytolytic effects of TNF 
      alpha and IL-1 alpha, ME180 cells were sensitive. EC were also less sensitive to 
      TNF alpha than ME180 cells, as judged by viability of individual cells and by the 
      release of lactate dehydrogenase (LDH) into the medium. Both manganese superoxide 
      dismutase (Mn-SOD) and its mRNA were markedly induced by these cytokines in EC 
      and in A549 cells but not in ME180 cells. The levels of Mn-SOD in the conditioned 
      medium of EC were markedly increased after stimulation with cytokines, whereas 
      those in ME180 and A549 cells were relatively low. The amount of Mn-SOD released 
      appears to be comparable to that from cells lysed due to the cytocidal effect of 
      cytokines, as assessed by measuring intra- and extra-cellular LDH activity. These 
      data suggest that, in vivo, the TNF alpha and IL-1 alpha produced by cancer cells 
      and other cells may induce Mn-SOD in vascular endothelial cells as well as other 
      host tissues, resulting in release of a relatively large amount of this protein 
      into the serum.
FAU - Nakata, T
AU  - Nakata T
AD  - Department of Biochemistry, Osaka University Medical School, Japan.
FAU - Suzuki, K
AU  - Suzuki K
FAU - Fujii, J
AU  - Fujii J
FAU - Ishikawa, M
AU  - Ishikawa M
FAU - Taniguchi, N
AU  - Taniguchi N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Blotting, Northern
MH  - Culture Media, Conditioned
MH  - Endothelium/*enzymology
MH  - Enzyme Induction
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Mitochondria/*enzymology
MH  - Superoxide Dismutase/*biosynthesis
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Umbilical Veins/enzymology/ultrastructure
EDAT- 1993/10/21 00:00
MHDA- 1993/10/21 00:01
CRDT- 1993/10/21 00:00
PHST- 1993/10/21 00:00 [pubmed]
PHST- 1993/10/21 00:01 [medline]
PHST- 1993/10/21 00:00 [entrez]
AID - 10.1002/ijc.2910550422 [doi]
PST - ppublish
SO  - Int J Cancer. 1993 Oct 21;55(4):646-50. doi: 10.1002/ijc.2910550422.