PMID- 8407966
OWN - NLM
STAT- MEDLINE
DCOM- 19931124
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 268
IP  - 28
DP  - 1993 Oct 5
TI  - Novel isozymes of cAMP-dependent protein kinase exist in human cells due to 
      formation of RI alpha-RI beta heterodimeric complexes.
PG  - 21276-83
AB  - We report that a human neoplastic B cell line (Reh) contains cAMP-dependent 
      protein kinase (cAK) type I (cAKI), but is practically devoid of cAK type II 
      (cAKII). However, these cells contain a novel cAKI isozyme consisting of an RI 
      alpha-RI beta heterodimer in association with phosphotransferase activity (RI 
      alpha RI beta C2) eluting from DEAE-cellulose columns at a salt concentration 
      characteristic of a cAKII. Immunoprecipitation of 8-azido-[32P]cAMP-labeled 
      extracts and DEAE fractions employing specific antibodies directed against RI 
      alpha and RI beta clearly demonstrated the presence of RI alpha-RI beta 
      heterodimers. RI alpha was precipitated with RI beta antiserum and vice versa. 
      Furthermore, disruption of disulfide bridges by reduction-alkylation abolished 
      this coimmunoprecipitation. In addition, formation of heterodimeric complexes of 
      RI alpha and RI beta could be demonstrated in vitro using recombinant RI 
      proteins. Finally, the presence of low levels of RI alpha-RI beta heterodimers 
      could also be demonstrated in human peripheral blood T lymphocytes. RI alpha-RI 
      beta heterodimers complexed with the catalytic subunit represent a novel isozyme 
      of cAKI (RI alpha RI beta C2), which enhances the possibilities for 
      diversification of cAMP-mediated effects.
FAU - Tasken, K
AU  - Tasken K
AD  - Institute of Medical Biochemistry, University of Oslo, Norway.
FAU - Skalhegg, B S
AU  - Skalhegg BS
FAU - Solberg, R
AU  - Solberg R
FAU - Andersson, K B
AU  - Andersson KB
FAU - Taylor, S S
AU  - Taylor SS
FAU - Lea, T
AU  - Lea T
FAU - Blomhoff, H K
AU  - Blomhoff HK
FAU - Jahnsen, T
AU  - Jahnsen T
FAU - Hansson, V
AU  - Hansson V
LA  - eng
GR  - GM34921/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Azides)
RN  - 0 (Cyclic AMP-Dependent Protein Kinase RIalpha Subunit)
RN  - 0 (Cyclic AMP-Dependent Protein Kinase RIbeta Subunit)
RN  - 0 (Isoenzymes)
RN  - 0 (PRKAR1A protein, human)
RN  - 0 (PRKAR1B protein, human)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - 31966-52-6 (8-azidoadenosine-3',5'-monophosphate)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinase Type II)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibody Specificity
MH  - Azides
MH  - B-Lymphocytes/*enzymology
MH  - Chromatography, DEAE-Cellulose
MH  - Cyclic AMP/analogs & derivatives
MH  - Cyclic AMP-Dependent Protein Kinase RIalpha Subunit
MH  - Cyclic AMP-Dependent Protein Kinase RIbeta Subunit
MH  - Cyclic AMP-Dependent Protein Kinase Type II
MH  - Cyclic AMP-Dependent Protein Kinases/chemistry/immunology/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Isoenzymes/*metabolism
MH  - Molecular Sequence Data
MH  - Peptide Fragments/chemistry/immunology
MH  - Recombinant Proteins/chemistry/immunology/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1993/10/05 00:00
MHDA- 1993/10/05 00:01
CRDT- 1993/10/05 00:00
PHST- 1993/10/05 00:00 [pubmed]
PHST- 1993/10/05 00:01 [medline]
PHST- 1993/10/05 00:00 [entrez]
AID - S0021-9258(19)36921-2 [pii]
PST - ppublish
SO  - J Biol Chem. 1993 Oct 5;268(28):21276-83.