PMID- 8409521
OWN - NLM
STAT- MEDLINE
DCOM- 19931104
LR  - 20190723
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 101
IP  - 4
DP  - 1993 Oct
TI  - Reciprocal cytokine-mediated cellular interactions in mouse epidermis: promotion 
      of gamma delta T-cell growth by IL-7 and TNF alpha and inhibition of keratinocyte 
      growth by gamma IFN.
PG  - 543-8
AB  - A unique subset of gamma delta T cells, termed dendritic epidermal T cells 
      (DETC), resides in symbiosis with keratinocytes in mouse epidermis. We have shown 
      previously that interleukin 7 (IL-7) which is produced by keratinocytes, promotes 
      growth and prevents apoptosis in DETC. To extend this observation, we examined 12 
      cytokines, each of which is expressed by epidermal cells at mRNA and/or protein 
      levels, for their capacities to modulate the growth of DETC. Cytokines examined 
      included IL-1 alpha, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, tumor necrosis 
      factor-alpha (TNF alpha), interferon-gamma (IFN gamma), 
      granulocyte/macrophage-colony stimulating factor (GM-CSF), and macrophage 
      inflammatory protein-1 alpha (MIP-1 alpha). When tested individually, IL-2 and 
      IL-7 promoted maximal growth of the long-term cultured DETC line 7-17. When 
      tested in combinations, synergistic growth-promoting effects were seen with IL-2 
      and IL-4 or IL-7, and with IL-7 and IL-4 or TNF alpha. Dose-response experiments 
      demonstrated that TNF alpha, which is produced by keratinocytes, enhances 
      IL-7-induced DETC proliferation, but inhibits IL-2-induced proliferation. The 
      mouse keratinocyte-derived cell line Pam 212 was used to test these cytokines for 
      their capacities to regulate keratinocyte growth. Only gamma IFN, which is 
      produced by DETC, inhibited proliferation in a dose-dependent fashion. These 
      results illustrate three reciprocal pathways by which epidermal cytokines 
      regulate the growth of epidermal cells: 1) a paracrine mechanism by which 
      keratinocyte-derived cytokines (e.g., IL-7 and TNF alpha) promote the growth of 
      DETC, 2) an autocrine mechanism by which DETC-derived cytokines (e.g., IL-2 and 
      IL-4) support their own growth, and 3) a reciprocal pathway in which a cytokine 
      produced by resident epidermal leukocytes (e.g., gamma IFN) modulates the growth 
      of keratinocytes.
FAU - Matsue, H
AU  - Matsue H
AD  - Department of Dermatology, University of Texas Southwestern Medical Center, 
      Dallas 75235.
FAU - Bergstresser, P R
AU  - Bergstresser PR
FAU - Takashima, A
AU  - Takashima A
LA  - eng
GR  - R01-AR35068/AR/NIAMS NIH HHS/United States
GR  - R01-AR40042/AR/NIAMS NIH HHS/United States
GR  - R01-AR41150/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-2)
RN  - 0 (Interleukin-7)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - *Cell Communication
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cytokines/*pharmacology
MH  - Dexamethasone/antagonists & inhibitors/pharmacology
MH  - *Epidermal Cells
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-2/pharmacology
MH  - Interleukin-7/pharmacology
MH  - Keratinocytes/cytology
MH  - Mice
MH  - T-Lymphocyte Subsets/cytology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - S0022-202X(93)90404-6 [pii]
AID - 10.1111/1523-1747.ep12365938 [doi]
PST - ppublish
SO  - J Invest Dermatol. 1993 Oct;101(4):543-8. doi: 10.1111/1523-1747.ep12365938.