PMID- 8409948
OWN - NLM
STAT- MEDLINE
DCOM- 19931116
LR  - 20061115
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 74 ( Pt 10)
DP  - 1993 Oct
TI  - Monoclonal antibodies to glycoprotein B differentiate human herpesvirus 6 into 
      two clusters, variants A and B.
PG  - 2257-62
AB  - The distribution of glycoprotein B (gB) among different human herpesvirus 6 
      (HHV-6) strains was analysed with a panel of three monoclonal antibodies (MAbs) 
      derived from mice immunized with U1102-infected lymphocytes. MAb 2D9 reacted 
      specifically by immunofluorescence and immunoprecipitation with the U1102 and GS 
      isolates, and failed to react with Z29 and the variant B strains Hashimoto and 
      SF. In addition, Z29, Hashimoto and SF gB had a lower M(r) than U1102 and GS gB. 
      MAb 2D9 also failed to react with the exanthem subitum isolate CV, included in 
      this study as an as yet poorly characterized isolate. Consistent with this 
      result, CV failed to react with the variant A-specific MAb to gp82-105 and 
      behaved as a variant B virus even with respect to the diagnostic HindIII 
      endonuclease restriction cleavage site located in a fragment hybridizing to the 
      pZVH14 probe. By contrast with MAb 2D9, MAbs 2B9 and 2D10 reacted with all of the 
      isolates tested, strengthening the argument tha they have common epitopes. Based 
      on the antigenic and M(r) specificities of gB, the HHV-6 isolates tested were 
      arranged into two non-overlapping clusters, which closely parallel the variant A 
      and B strain groups, defined previously by several criteria, including 
      restriction endonuclease polymorphism, antigenic variations, growth in in vitro 
      cultures and sequence analyses.
FAU - Campadelli-Fiume, G
AU  - Campadelli-Fiume G
AD  - Department of Experimental Pathology, University of Bologna, Italy.
FAU - Guerrini, S
AU  - Guerrini S
FAU - Liu, X
AU  - Liu X
FAU - Foa-Tomasi, L
AU  - Foa-Tomasi L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 148770-71-2 (glycoprotein B, human herpesvirus-6)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigenic Variation
MH  - Antigens, Viral/*analysis
MH  - Herpesvirus 6, Human/classification/*immunology
MH  - Humans
MH  - Viral Envelope Proteins/*analysis/immunology
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 10.1099/0022-1317-74-10-2257 [doi]
PST - ppublish
SO  - J Gen Virol. 1993 Oct;74 ( Pt 10):2257-62. doi: 10.1099/0022-1317-74-10-2257.