PMID- 8410187
OWN - NLM
STAT- MEDLINE
DCOM- 19931112
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 13
IP  - 10
DP  - 1993 Oct
TI  - Signal transduction events mediated by the BDNF receptor gp 145trkB in primary 
      hippocampal pyramidal cell culture.
PG  - 4281-92
AB  - The trkB gene encodes a tyrosine kinase receptor, gp145trkB, for brain-derived 
      neurotrophic factor (BDNF) and neurotrophin-4 (NT-4). To understand the role of 
      gp145trkB in the nervous system, we have investigated its expression in embryonic 
      rat hippocampal pyramidal cell cultures and examined the effects of BDNF on 
      signal transduction in the primary neurons. The expression of trkB transcripts 
      was established by PCR analysis and in situ hybridization. In addition to 
      gp145trkB, the pyramidal neuronal cultures expressed transcripts specific for the 
      NT-3 receptor gp145trkC, but not for the high-affinity NGF receptor gp140trk or 
      for p75LNGFR, a low-affinity receptor for all known members of the NGF family of 
      neurotrophins including the gp145trkB ligands, BDNF and NT-4. The presence of 
      gp145trkB receptors in the primary neuronal cultures was confirmed by 
      immunocytochemical analysis in which > 90% of the cells stained with 
      affinity-purified polyclonal antibodies to gp145trkB. Immunoblots using this 
      antibody revealed a single approximately 140 kDa protein in both adult 
      hippocampus and pyramidal cultures. Addition of recombinant BDNF to these 
      cultures induced the tyrosine phosphorylation of gp145trkB, as determined by 
      antiphosphotyrosine staining of gp145trkB immunoprecipitates. Moreover, BDNF 
      treatment activated the microtubule-associated protein (MAP) kinases, as 
      determined by an increase in MAP2 phosphorylation in vitro. Both the 41 and 44 
      kDa forms of MAP kinase were activated by BDNF. BDNF also increased c-fos 
      expression in over 90% of the cells. These results indicate that gp145trkB does 
      not require p75LNGFR to form a functional receptor for BDNF in hippocampal 
      pyramidal neurons.
FAU - Marsh, H N
AU  - Marsh HN
AD  - Department of Pharmacological and Physiological Sciences, University of Chicago, 
      Illinois 60637.
FAU - Scholz, W K
AU  - Scholz WK
FAU - Lamballe, F
AU  - Lamballe F
FAU - Klein, R
AU  - Klein R
FAU - Nanduri, V
AU  - Nanduri V
FAU - Barbacid, M
AU  - Barbacid M
FAU - Palfrey, H C
AU  - Palfrey HC
LA  - eng
GR  - GM-42715/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA Primers)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Ciliary Neurotrophic Factor)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (oncogene protein trk)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
GS  - trk
GS  - trkB
GS  - trkC
MH  - 3T3 Cells
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/drug effects/*physiology
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Cells, Cultured
MH  - DNA Primers
MH  - Enzyme Activation
MH  - Gene Expression
MH  - Hippocampus/*physiology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Kinetics
MH  - Membrane Proteins/analysis/biosynthesis
MH  - Mice
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Nerve Growth Factors/pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neurons/drug effects/*physiology
MH  - Oncogene Proteins/analysis/biosynthesis/*metabolism
MH  - Polymerase Chain Reaction
MH  - Pyramidal Tracts/drug effects/*physiology
MH  - RNA, Messenger/analysis/metabolism
MH  - Rats
MH  - Receptor, Ciliary Neurotrophic Factor
MH  - Receptors, Growth Factor/analysis/biosynthesis/*metabolism
MH  - Signal Transduction/drug effects/*physiology
PMC - PMC6576374
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
PMCR- 1994/04/01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
PHST- 1994/04/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.13-10-04281.1993 [doi]
PST - ppublish
SO  - J Neurosci. 1993 Oct;13(10):4281-92. doi: 10.1523/JNEUROSCI.13-10-04281.1993.