PMID- 8411380
OWN - NLM
STAT- MEDLINE
DCOM- 19931124
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 67
IP  - 11
DP  - 1993 Nov
TI  - Activation of the Epstein-Barr virus replicative cycle by human herpesvirus 6.
PG  - 6768-77
AB  - One common attribute of herpesviruses is the ability to establish latent, 
      life-long infections. The role of virus-virus interaction in viral reactivation 
      between or among herpesviruses has not been studied. Preliminary experiments in 
      our laboratory had indicated that infection of Epstein-Barr virus (EBV) 
      genome-positive human lymphoid cell lines with human herpesvirus 6 (HHV-6) 
      results in EBV reactivation in these cells. To further our knowledge of this 
      complex phenomenon, we investigated the effect of HHV-6 infection on expression 
      of the viral lytic cycle proteins of EBV. Our results indicate that HHV-6 
      upregulates, by up to 10-fold, expression of the immediate-early Zebra antigen 
      and the diffuse and restricted (85 kDa) early antigens (EA-D and EA-R, 
      respectively) in both EBV producer and nonproducer cell lines (i.e., P3HR1, 
      Akata, and Raji). Maximal EA-D induction was observed at 72 h post-HHV-6 
      infection. Furthermore, expression of late EBV gene products, namely, the viral 
      capsid antigen (125 kDa) and viral membrane glycoprotein gp350, was also 
      increased in EBV producer cells (P3HR1 and Akata) following infection by HHV-6. 
      By using dual-color membrane immunofluorescence, it was found that most of the 
      cells expressing viral membrane glycoprotein gp350 were also positive for HHV-6 
      antigens, suggesting a direct effect of HHV-6 replication on induction of the EBV 
      replicative cycle. No expression of late EBV antigens was observed in Raji cells 
      following infection by HHV-6, implying a lack of functional complementation 
      between the deleted form of EBV found in Raji cells and the superinfecting HHV-6. 
      The susceptibility of the cell lines to infection by HHV-6 correlated with 
      increased expression of various EBV proteins in that B95-8 cells, which are not 
      susceptible to HHV-6 infection, did not show an increase in expression of EBV 
      antigens following treatment with HHV-6. Moreover, UV light-irradiated or 
      heat-inactivated HHV-6 had no upregulating effect on the Zebra antigen or EA-D in 
      Raji cells, indicating that infectious virus is required for the observed effects 
      of HHV-6 on these EBV products. These results show that HHV-6, another 
      lymphotropic human herpesvirus, can activate EBV replication and may thus 
      contribute to the pathogenesis of EBV-associated diseases.
FAU - Flamand, L
AU  - Flamand L
AD  - Laboratory of Immunovirology, Ste-Justine Hospital, Montreal, Canada.
FAU - Stefanescu, I
AU  - Stefanescu I
FAU - Ablashi, D V
AU  - Ablashi DV
FAU - Menezes, J
AU  - Menezes J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, Viral)
RN  - 0 (BZLF1 protein, Herpesvirus 4, Human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Epstein-Barr virus early antigen)
RN  - 0 (Epstein-Barr virus early antigen diffuse component)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Antigens, Viral/*metabolism
MH  - Cell Line
MH  - DNA-Binding Proteins/metabolism
MH  - Gene Expression Regulation, Viral
MH  - Herpesvirus 4, Human/*growth & development/immunology
MH  - Herpesvirus 6, Human/*growth & development
MH  - Humans
MH  - In Vitro Techniques
MH  - Membrane Glycoproteins/metabolism
MH  - Trans-Activators/metabolism
MH  - Viral Proteins/metabolism
MH  - *Virus Replication
PMC - PMC238118
EDAT- 1993/11/01 00:00
MHDA- 1993/11/01 00:01
PMCR- 1993/11/01
CRDT- 1993/11/01 00:00
PHST- 1993/11/01 00:00 [pubmed]
PHST- 1993/11/01 00:01 [medline]
PHST- 1993/11/01 00:00 [entrez]
PHST- 1993/11/01 00:00 [pmc-release]
AID - 10.1128/JVI.67.11.6768-6777.1993 [doi]
PST - ppublish
SO  - J Virol. 1993 Nov;67(11):6768-77. doi: 10.1128/JVI.67.11.6768-6777.1993.