PMID- 8415539
OWN - NLM
STAT- MEDLINE
DCOM- 19931028
LR  - 20190909
IS  - 0932-0113 (Print)
IS  - 0932-0113 (Linking)
VI  - 79
IP  - 5
DP  - 1993
TI  - Entamoeba histolytica: changes in the zymodeme of cloned nonpathogenic 
      trophozoites cultured under different conditions.
PG  - 353-6
AB  - In this paper we report the occurrence of changes in the migration of certain 
      isoenzymes of a cloned strain (MAV-CINVESTAV) of Entamoeba histolytica. This 
      strain was isolated from an asymptomatic carrier and showed an initial 
      nonpathogenic zymodeme I. The transfer of polyxenic trophozoites from Robinson's 
      medium (7% serum) to Jones' medium (30% serum) provoked changes in isoenzyme 
      expression, resulting in the conversion of zymodeme I to a zymodeme that was 
      similar to the XVII zymodeme except for two slow-running bands and a single 
      fast-running band that were detected for hexokinase (HK). This XVII-like zymodeme 
      reverted to zymodeme I when trophozoites were cultured under monoxenic conditions 
      in TY1-S-33 medium (10% serum). When we cultured cloned strain MAV-CINVESTAV 
      under axenic conditions in TY1-S-33 medium, trophozoites expressed a pathogenic 
      zymodeme with two fast-running HK bands and a beta-phosphoglucomutase band. In 
      addition, phagocytosis and the ability of the trophozoites to destroy 
      cell-culture monolayers were expressed only in trophozoites cultured under axenic 
      conditions. The axenization procedure required the presence of live Fusobacterium 
      symbiosum and is also described herein.
FAU - Vargas, M A
AU  - Vargas MA
AD  - Department of Experimental Pathology, CINVESTAV I.P.N., Mexico, D.F.
FAU - Orozco, E
AU  - Orozco E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Parasitol Res
JT  - Parasitology research
JID - 8703571
RN  - 0 (Isoenzymes)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 5.4.2.2 (Phosphoglucomutase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Entamoeba histolytica/classification/*enzymology/growth & 
      development/pathogenicity
MH  - Hexokinase/*genetics/metabolism
MH  - Isoenzymes/*genetics/metabolism
MH  - Oxygen/metabolism
MH  - Phenotype
MH  - Phosphoglucomutase/*genetics/metabolism
MH  - Species Specificity
MH  - Virulence
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1007/BF00931822 [doi]
PST - ppublish
SO  - Parasitol Res. 1993;79(5):353-6. doi: 10.1007/BF00931822.