PMID- 8417807
OWN - NLM
STAT- MEDLINE
DCOM- 19930203
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 81
IP  - 1
DP  - 1993 Jan 1
TI  - Human T-lymphotropic virus type I tax regulates the expression of the human 
      lymphotoxin gene.
PG  - 95-100
AB  - Human T-lymphotropic virus type-I (HTLV-I)-infected T-cell lines constitutively 
      produce high levels of lymphotoxin (LT). To analyze the mechanisms that lead to 
      the expression of LT in HTLV-I-infected cell lines, we studied regulatory regions 
      of the human LT promoter involved in the activation of the human LT gene. As 
      determined by deletional analysis, sequences between +137 and -116 (relative to 
      the transcription initiation site) are sufficient to direct expression of a 
      reporter gene in the HTLV-I-infected cell line MT-2. Site-directed mutation of a 
      of the single kappa B-like motif present in the LT promoter region (positions -99 
      to -89) completely abrogated LT promoter activity in MT-2 cells, suggesting that 
      this site plays a critical role in the activation of the human LT gene. 
      Transfection of LT constructs into HTLV-I-uninfected and -unstimulated Jurkat and 
      U937 cell lines showed little to no activity of the LT promoter. Cotransfection 
      of the same constructs with a tax expression plasmid into Jurkat cells led to 
      detectable promoter activity, which could be significantly increased by 
      stimulation of the cells with phorbol myristate acetate (PMA). Similarly, 
      cotransfection of the LT promoter constructs and the tax expression plasmid into 
      U937 cells led to significant promoter activity upon stimulation with PMA. These 
      data suggest that HTLV-I tax is involved in the upregulation of LT gene 
      expression in HTLV-I-infected cells.
FAU - Tschachler, E
AU  - Tschachler E
AD  - Department of Dermatology, University of Vienna Medical School, Austria.
FAU - Bohnlein, E
AU  - Bohnlein E
FAU - Felzmann, S
AU  - Felzmann S
FAU - Reitz, M S Jr
AU  - Reitz MS Jr
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (NF-kappa B)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
GS  - tax
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Line
MH  - *Gene Expression Regulation
MH  - *Genes, pX
MH  - Human T-lymphotropic virus 1/*genetics
MH  - Humans
MH  - Lymphotoxin-alpha/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - NF-kappa B/metabolism
MH  - Plasmids
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic
MH  - Regulatory Sequences, Nucleic Acid
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transfection
EDAT- 1993/01/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - S0006-4971(20)73300-8 [pii]
PST - ppublish
SO  - Blood. 1993 Jan 1;81(1):95-100.