PMID- 8418206
OWN - NLM
STAT- MEDLINE
DCOM- 19930201
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 177
IP  - 1
DP  - 1993 Jan 1
TI  - Human neutrophils express immunoglobulin E (IgE)-binding proteins (Mac-2/epsilon 
      BP) of the S-type lectin family: role in IgE-dependent activation.
PG  - 243-8
AB  - It has been suggested that neutrophils may be involved in the late-phase reaction 
      of immunoglobulin E (IgE)-dependent hypersensitivity states. However, the 
      identity of neutrophil-associated molecules inducing the release of mediators 
      remains unclear. In this report, we demonstrate that human neutrophils from 
      normal donors or from patients with inflammatory disorders could bind myeloma IgE 
      proteins, especially after desialylation. Northern blot, immunoprecipitation, and 
      flow cytometry analyses revealed that neutrophils did not express Fc epsilon 
      RII/CD23, but rather Mac-2/epsilon binding protein (BP), belonging to the S-type 
      lectin family. Similarly to IgA used as positive control, myeloma IgE proteins, 
      as well as polyclonal IgE antibodies with or without antibody specificity, were 
      both capable of inducing a neutrophil respiratory burst. Anti-Mac-2 but not 
      anti-CD23 mAb strongly decreased the IgE-dependent activation of neutrophils, 
      induced either by the specific antigen or by anti-IgE antibodies. These findings 
      open new perspectives on the functional role of neutrophils in IgE-associated 
      diseases including allergic states or parasitic infections.
FAU - Truong, M J
AU  - Truong MJ
AD  - Centre d'Immunologie et de Biologie Parasitaire (CIBP), Institut Pasteur, Lille, 
      France.
FAU - Gruart, V
AU  - Gruart V
FAU - Kusnierz, J P
AU  - Kusnierz JP
FAU - Papin, J P
AU  - Papin JP
FAU - Loiseau, S
AU  - Loiseau S
FAU - Capron, A
AU  - Capron A
FAU - Capron, M
AU  - Capron M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Galectin 3)
RN  - 0 (RNA, Messenger)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antigens, Differentiation/*analysis/genetics/physiology
MH  - Flow Cytometry
MH  - Galectin 3
MH  - Humans
MH  - Immunoglobulin E/*physiology
MH  - Multiple Myeloma/immunology
MH  - Neutrophils/*immunology/physiology
MH  - Precipitin Tests
MH  - RNA, Messenger/analysis
PMC - PMC2190867
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
PMCR- 1993/07/01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
PHST- 1993/07/01 00:00 [pmc-release]
AID - 93115668 [pii]
AID - 10.1084/jem.177.1.243 [doi]
PST - ppublish
SO  - J Exp Med. 1993 Jan 1;177(1):243-8. doi: 10.1084/jem.177.1.243.