PMID- 8420396
OWN - NLM
STAT- MEDLINE
DCOM- 19930208
LR  - 20071114
IS  - 0003-276X (Print)
IS  - 0003-276X (Linking)
VI  - 235
IP  - 2
DP  - 1993 Feb
TI  - Dendritic cell/lymphocyte clustering: morphologic analysis by transmission 
      electron microscopy and distribution of gold-labeled MHC class II antigens by 
      high-resolution scanning electron microscopy.
PG  - 285-95
AB  - Dendritic cells (DCs) are potent antigen-presenting cells for a variety of immune 
      responses; however, their mechanism of action has not been established. It is 
      known that DCs can cluster with one another and with other cell types during in 
      vitro immune responses, and clustering may be essential for the activation of 
      resting lymphocytes. In this study, ultrastructural examination of clusters that 
      form during extended culture of enriched rat splenic DCs (approximately 70% DCs) 
      is reported. DCs were readily distinguished from other cell types, which included 
      lymphocytes and macrophages. DCs displayed characteristic veils and/or dendritic 
      processes that intertwined with processes of other cells within the cluster, or 
      extended from the cluster periphery. Occasional DCs contained large vacuoles 
      lined with small vesicles. A paramount feature of DCs is their constitutive 
      expression of high levels of surface major histocompatibility complex class II 
      antigens. The surface distribution of class II antigens on clustering DCs was 
      examined using 10 nm immunogold labeling techniques and high-resolution scanning 
      electron microscopy. DCs were readily distinguished by morphologic criteria, and 
      examination of various surface membrane regions revealed a differential 
      distribution of class II antigens. Gold label was frequently distributed in 
      linear arrays and clusters, suggesting a cytoskeletal role in the 
      recycling/redistribution of Class II antigens. These morphologic findings further 
      an understanding of basic DC biology and their mechanism of action as 
      antigen-presenting cells.
FAU - Setum, C M
AU  - Setum CM
AD  - Department of Cell Biology and Neuroanatomy, University of Minnesota, 
      Minneapolis.
FAU - Serie, J R
AU  - Serie JR
FAU - Hegre, O D
AU  - Hegre OD
LA  - eng
GR  - DK32237/DK/NIDDK NIH HHS/United States
GR  - DK37436/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Anat Rec
JT  - The Anatomical record
JID - 0370540
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Animals
MH  - Cell Communication
MH  - Dendritic Cells/chemistry/*ultrastructure
MH  - Histocompatibility Antigens Class II/*analysis
MH  - Lymphocytes/ultrastructure
MH  - Male
MH  - Microscopy, Electron
MH  - Microscopy, Electron, Scanning
MH  - Rats
MH  - Rats, Inbred F344
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
AID - 10.1002/ar.1092350212 [doi]
PST - ppublish
SO  - Anat Rec. 1993 Feb;235(2):285-95. doi: 10.1002/ar.1092350212.