PMID- 8420805
OWN - NLM
STAT- MEDLINE
DCOM- 19930218
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 316
IP  - 2
DP  - 1993 Jan 25
TI  - Characterization of metabotropic glutamate receptors coupled to a pertussis toxin 
      sensitive G-protein in bovine brain coated vesicles.
PG  - 191-6
AB  - Glutamate metabotropic receptors (mGluRs) in bovine brain coated vesicles have 
      been characterized by pharmacological and kinetic binding experiments. Saturation 
      experiments revealed a single binding site with a Kd = 607.9 +/- 78.5 nM and a 
      Bmax = 6.45 +/- 0.88 pmol/mg protein. The specific binding of L-[3H]glutamate to 
      mGluRs is regulated by guanine nucleotides. Guanosine-5'-triphosphate (GTP; 100 
      microM) shifts the agonist competition curves to the right, increasing the IC50 
      values. Pertussis toxin treatment produces a pharmacological binding profile for 
      quisqualate similar to that obtained in the presence of 100 microM GTP. These 
      results indicate the presence of metabotropic glutamate receptors in coated 
      vesicles and its coupling to a pertussis toxin sensitive G-protein.
FAU - Martin, M
AU  - Martin M
AD  - Departamento de Inorganica, Organica y Bioquimica, Facultad de Quimicas, 
      Universidad de Castilla-La Mancha, Ciudad Real, Spain.
FAU - Sanz, J M
AU  - Sanz JM
FAU - Cubero, A
AU  - Cubero A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Receptors, Glutamate)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Cattle
MH  - Coated Pits, Cell-Membrane/*metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Guanosine Triphosphate/metabolism
MH  - *Pertussis Toxin
MH  - Radioligand Assay
MH  - Receptors, Glutamate/*metabolism
MH  - Virulence Factors, Bordetella/*metabolism
EDAT- 1993/01/25 00:00
MHDA- 1993/01/25 00:01
CRDT- 1993/01/25 00:00
PHST- 1993/01/25 00:00 [pubmed]
PHST- 1993/01/25 00:01 [medline]
PHST- 1993/01/25 00:00 [entrez]
AID - 0014-5793(93)81213-J [pii]
AID - 10.1016/0014-5793(93)81213-j [doi]
PST - ppublish
SO  - FEBS Lett. 1993 Jan 25;316(2):191-6. doi: 10.1016/0014-5793(93)81213-j.