PMID- 8426737
OWN - NLM
STAT- MEDLINE
DCOM- 19930226
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 8
IP  - 2
DP  - 1993 Feb
TI  - Tumor suppressor activity of RB and p53 genes in human breast carcinoma cells.
PG  - 279-88
AB  - Breast cancer is the most common cancer of women in Western countries. Various 
      genetic alterations have been implicated in its development. Two tumor suppressor 
      genes, the retinoblastoma susceptibility gene (RB) and the gene encoding the p53 
      protein, are frequently found to be deleted or mutated in breast cancer cell 
      lines and primary tumor samples. Breast carcinoma cell lines MDA-MB468 and BT549 
      both harbor partial RB gene deletions as well as point mutations of their p53 
      genes, thus providing an excellent model system for testing the roles played by 
      these two genes in the oncogenesis of breast cancer. Single copies of wild-type 
      RB or p53 were delivered to these cells by retrovirus-mediated gene transfer. 
      Restoration of RB expression in cells reduced their ability to grow in soft agar 
      and their tumorigenicity in nude mice, although no significant alteration of 
      growth rate in culture could be detected. Introduction of wild-type p53 into 
      these cells exerted a similar effect on the neoplastic phenotypes. This effect 
      occurred even in the presence of their endogenous mutated p53 alleles, which 
      argues for the phenotypical dominance of the wild-type p53 over mutated p53 
      during human oncogenesis. The ability of RB and p53 genes to suppress the 
      tumorigenicity of breast carcinoma cells provides functional evidence that 
      deletion or mutational inactivation of tumor suppressor genes represents an 
      important step in the genesis of breast cancer.
FAU - Wang, N P
AU  - Wang NP
AD  - Center for Molecular Medicine, University of Texas Health Science Center, San 
      Antonio 78245.
FAU - To, H
AU  - To H
FAU - Lee, W H
AU  - Lee WH
FAU - Lee, E Y
AU  - Lee EY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
SB  - IM
GS  - RB
GS  - p53
MH  - Animals
MH  - Breast Neoplasms/*genetics/pathology/prevention & control
MH  - Female
MH  - *Genes, Retinoblastoma
MH  - *Genes, p53
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Tumor Cells, Cultured
MH  - Virus Integration
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1993 Feb;8(2):279-88.