PMID- 8428796
OWN - NLM
STAT- MEDLINE
DCOM- 19930308
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 53
IP  - 3
DP  - 1993 Feb 1
TI  - Monocyte-conditioned media possess a novel factor which increases motility of 
      cancer cells.
PG  - 426-31
AB  - A number of cytokines have been reported to increase the cell motility (scatter) 
      of cohesive cell colonies. We report here a novel scattering factor produced by 
      human monocytes. Media from both stimulated and non-stimulated human monocytes or 
      the monocytic cell line, U937, increased the cell motility of 2 human 
      colon-cancer cell lines, HT115 and HT29, but not the canine epithelial cell line 
      MDCK. Motility was assayed by cell dissociation from carrier beads and colony 
      scattering. HT115 cells were strongly scattered by the conditioned media but not 
      by interleukins IL-1, 2, 4, 6, 8, 10, TNF alpha, TGF beta, EGF, GM-CSF, IGF-I, 
      PDGF, interferon-gamma. This factor is distinct from hepatocyte growth 
      factor/scatter factor (HGF/SF), since the activity was not blocked by anti-HGF/SF 
      antibody. The activity was reduced by treatment with acid, heat, trypsin and 
      dithiothreitol; this, together with gel filtration, suggests that the factor is a 
      protein (MW 40 to 60 kDa). This new factor, which is secreted from monocytes and 
      the monocytic cell line, U937, has the ability to increase the motility of cancer 
      cells and may be important in controlling the behaviour of tumours in vivo.
FAU - Jiang, W G
AU  - Jiang WG
AD  - University Department of Surgery, University of Wales, College of Medicine, 
      Cardiff, UK.
FAU - Puntis, M C
AU  - Puntis MC
FAU - Hallett, M B
AU  - Hallett MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Culture Media)
RN  - 0 (Cytokines)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - *Cell Movement
MH  - Culture Media
MH  - Cytokines/*physiology
MH  - Dogs
MH  - Hepatocyte Growth Factor/physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Monocytes/*physiology
MH  - Tumor Cells, Cultured/pathology
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
AID - 10.1002/ijc.2910530313 [doi]
PST - ppublish
SO  - Int J Cancer. 1993 Feb 1;53(3):426-31. doi: 10.1002/ijc.2910530313.