PMID- 8429622
OWN - NLM
STAT- MEDLINE
DCOM- 19930308
LR  - 20131121
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 17
IP  - 1
DP  - 1993 Jan-Feb
TI  - Fluorescence polarization immunoassay detection of amphetamine, methamphetamine, 
      and illicit amphetamine analogues.
PG  - 23-33
AB  - The Abbott Diagnostics Amphetamine/Methamphetamine II and Amphetamine Class 
      reagents were evaluated on the Abbott TDx for cross-reactivity to amphetamine and 
      methamphetamine stereoisomers, several of their metabolites, and various illicit 
      analogues, including 2-methoxyamphetamine, 4-hydroxymethamphetamine, 
      2,5-dimethoxy-amphetamine (DMA), 4-bromo-2,5-dimethoxyamphetamine (DOB), 
      4-bromo-2,5-dimethoxy-beta-phenethylamine (BDMPEA), 3,4,5-trimethoxyamphetamine 
      (TMA), 3,4-methylenedioxy-amphetamine (MDA) 
      N,N-dimethyl-3,4-methylenedioxy-amphetamine, 
      N-hydroxy-3,4-methylenedioxyamphetamine (N-OH MDA), 
      3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethylamphetamine 
      (MDEA), 2,5-dimethoxy-4-ethylamphetamine (DOE), 2,5-dimethoxy-4-methylamphetamine 
      (DOM), and mescaline in concentrations ranging from 100 to 100,000 ng/mL. Results 
      demonstrate the utility of this assay for detection of several of the above 
      compounds; unfortunately many are still not detectable. Significant differences 
      were observed between the Amphetamine/Methamphetamine II and Amphetamine Class 
      reagents, particularly regarding their cross-reactivity to over-the-counter 
      medications. Detection of the drugs amphetamine, methamphetamine, and the illicit 
      analogues is not enhanced with the Amphetamine Class reagents, and unless 
      detection of the over-the-counter compounds is of interest, these reagents are a 
      poor choice compared to the Amphetamine/Methamphetamine II reagents. 
      Cross-reactivity of some of the illicit analogues is such that the assay can 
      reliably be used for the routine screening of these compounds.
FAU - Cody, J T
AU  - Cody JT
AD  - Clinical Investigation Directorate, Wilford Hall Medical Center, Lackland AFB, TX 
      78236-5300.
FAU - Schwarzhoff, R
AU  - Schwarzhoff R
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Amphetamines)
RN  - 44RAL3456C (Methamphetamine)
SB  - IM
MH  - Amphetamines/*analysis/immunology
MH  - Antibody Specificity
MH  - Calibration
MH  - Cross Reactions
MH  - *Fluorescence Polarization Immunoassay
MH  - Humans
MH  - Methamphetamine/*analysis/immunology
MH  - Stereoisomerism
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
PST - ppublish
SO  - J Anal Toxicol. 1993 Jan-Feb;17(1):23-33.