PMID- 8430950
OWN - NLM
STAT- MEDLINE
DCOM- 19930309
LR  - 20181130
IS  - 0003-0805 (Print)
IS  - 0003-0805 (Linking)
VI  - 147
IP  - 2
DP  - 1993 Feb
TI  - Elevated release of tumor necrosis factor-alpha and interferon-gamma by 
      bronchoalveolar leukocytes from patients with bronchial asthma.
PG  - 291-5
AB  - Bronchoalveolar lavage (BAL) leukocyte-secreted cytokines are considered to be 
      important mediators of the inflammatory and allergic reactions in the lung. This 
      study examines quantitative changes in the level of tumor necrosis factor-alpha 
      (TNF alpha) and interferon-gamma (IFN gamma) production in BAL cell cultures 
      derived from patients (n = 11) with bronchial asthma. The secretion of TNF alpha 
      and IFN gamma was determined in intact (unstimulated) and 
      phytohemagglutinin/phorbol myristate acetate (PHA + PMA)-stimulated BAL leukocyte 
      cultures and compared with that in control cultures. In all patients studied, the 
      background and PHA + PMA-induced secretion of TNF alpha and IFN gamma was 
      significantly (p < 0.001) higher than that in parallel control cultures. In 
      contrast to BAL cell preparations, the capacity of TNF alpha and IFN gamma 
      secretion by patients' peripheral blood mononuclear cells (PBMC) did not differ 
      from that of control subjects. High spontaneous release of TNF alpha and IFN 
      gamma by patients' BAL leukocytes, but not PBMC, suggest that in the 
      pathophysiology of bronchial asthma, these cytokines may act as local pathogenic 
      agents in the lung.
FAU - Cembrzynska-Nowak, M
AU  - Cembrzynska-Nowak M
AD  - Laboratory of Virology, Polish Academy of Sciences, Wroclaw.
FAU - Szklarz, E
AU  - Szklarz E
FAU - Inglot, A D
AU  - Inglot AD
FAU - Teodorczyk-Injeyan, J A
AU  - Teodorczyk-Injeyan JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am Rev Respir Dis
JT  - The American review of respiratory disease
JID - 0370523
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Asthma/etiology/*physiopathology
MH  - Bronchoalveolar Lavage Fluid/*cytology
MH  - Bronchoscopy/methods
MH  - Cells, Cultured/chemistry/metabolism
MH  - Humans
MH  - Interferon-gamma/analysis/*metabolism
MH  - Leukocytes/chemistry/*metabolism
MH  - Leukocytes, Mononuclear/chemistry/metabolism
MH  - Middle Aged
MH  - Tumor Necrosis Factor-alpha/analysis/*metabolism
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
AID - 10.1164/ajrccm/147.2.291 [doi]
PST - ppublish
SO  - Am Rev Respir Dis. 1993 Feb;147(2):291-5. doi: 10.1164/ajrccm/147.2.291.