PMID- 8432209
OWN - NLM
STAT- MEDLINE
DCOM- 19930316
LR  - 20041117
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 14
IP  - 1
DP  - 1993
TI  - Detection of male cells in mixtures containing varying proportions of male and 
      female cells by fluorescence in situ hybridization and G-banding.
PG  - 9-15
AB  - The efficiency and sensitivity of fluorescence in situ hybridization (FISH) and 
      G-banding for the detection of male cells in mixtures of male and female cells 
      derived from peripheral blood (PB) and bone marrow (BM) are compared. Zero false 
      positive and negative rates were obtained with G-banding in all studies. False 
      positive rates obtained with FISH were low (0-2.5%) in both PB and BM. False 
      negative rates varied from 0-13.3%. Variations due to differences in fluorescent 
      counterstain or scorers were not significant and in some cases the percentage of 
      Y-positive cells as determined by FISH agreed more closely with the estimated 
      frequency of male cells than that obtained by G-banding. The minimum limits of 
      detection for FISH were 5% male cells on counting 500 PB or BM interphases; 10% 
      male cells on counting 100 PB metaphases; and 20% male cells on counting 50 BM 
      metaphases. This is in agreement with the theoretical limits of detection [95% 
      confidence limit (CL)]. However, minimum levels of detection for female cells 
      were below the 95% CL. It was not possible to reliably distinguish 95% female 
      cells from 100% on counting 500 PB or BM interphases; 90% from 100% on counting 
      100 PB metaphases; or 80% from 100% on counting 50 BM metaphases.
FAU - White, A D
AU  - White AD
AD  - Department of Haematology, University of Wales College of Medicine, Heath Park, 
      Cardiff, United Kingdom.
FAU - Sweeney, M C
AU  - Sweeney MC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
SB  - IM
MH  - Bone Marrow/ultrastructure
MH  - *Bone Marrow Cells
MH  - Chromosome Banding
MH  - Female
MH  - Genes
MH  - Humans
MH  - In Situ Hybridization
MH  - Lymphocytes/*cytology/ultrastructure
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Y Chromosome/*chemistry
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1002/cyto.990140104 [doi]
PST - ppublish
SO  - Cytometry. 1993;14(1):9-15. doi: 10.1002/cyto.990140104.