PMID- 8432525 OWN - NLM STAT- MEDLINE DCOM- 19930316 LR - 20190904 IS - 0888-7543 (Print) IS - 0888-7543 (Linking) VI - 15 IP - 1 DP - 1993 Jan TI - Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 genes or DNA markers. PG - 133-45 AB - We report here the band location of 540 cosmids mapped to chromosome 19. The cosmids were mapped by fluorescence in situ hybridization (FISH) relative to chromosomal bands produced by DAPI/actinomycin staining. The cosmids are distributed throughout the chromosome, with a sampling bias for the q-arm. A detailed analysis of the distribution of three different subtelomeric and 22 pericentromeric chromosome 19 cosmids on other chromosomes is also reported. Colony hybridization identified 142 cosmids that contain sequences representing genes or DNA markers that map to chromosome 19. FISH mapping of these cosmids sublocalizes a total of 70 genes and DNA markers on chromosome 19, revises the previously published map assignments of 2 genes, and narrows the location of over 20 markers. FAU - Trask, B AU - Trask B AD - Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California 94550. FAU - Fertitta, A AU - Fertitta A FAU - Christensen, M AU - Christensen M FAU - Youngblom, J AU - Youngblom J FAU - Bergmann, A AU - Bergmann A FAU - Copeland, A AU - Copeland A FAU - de Jong, P AU - de Jong P FAU - Mohrenweiser, H AU - Mohrenweiser H FAU - Olsen, A AU - Olsen A FAU - Carrano, A AU - Carrano A AU - et al. LA - eng GR - HG-00256/HG/NHGRI NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Genomics JT - Genomics JID - 8800135 RN - 0 (Genetic Markers) RN - 9007-49-2 (DNA) SB - IM MH - Animals MH - Centromere MH - *Chromosome Banding MH - Chromosome Mapping MH - *Chromosomes, Human, Pair 19 MH - *Cosmids MH - Cricetinae MH - DNA/genetics MH - *Genetic Markers MH - Humans MH - Hybrid Cells MH - In Situ Hybridization, Fluorescence MH - Telomere EDAT- 1993/01/01 00:00 MHDA- 1993/01/01 00:01 CRDT- 1993/01/01 00:00 PHST- 1993/01/01 00:00 [pubmed] PHST- 1993/01/01 00:01 [medline] PHST- 1993/01/01 00:00 [entrez] AID - S0888754383710219 [pii] AID - 10.1006/geno.1993.1021 [doi] PST - ppublish SO - Genomics. 1993 Jan;15(1):133-45. doi: 10.1006/geno.1993.1021.