PMID- 8432811
OWN - NLM
STAT- MEDLINE
DCOM- 19930316
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 31
IP  - 2
DP  - 1993 Feb
TI  - Evaluation of an immunoblot assay for serological confirmation and 
      differentiation of human T-cell lymphotropic virus types I and II.
PG  - 260-4
AB  - The confirmation of infection with human T-cell lymphotropic virus type I 
      (HTLV-I) and type II (HTLV-II) currently involves multiple assays. These include 
      Western blot (immunoblot) (WB) and/or radioimmunoprecipitation assay for 
      detection of antibodies to HTLV-specific viral proteins and polymerase chain 
      reaction and/or peptide-based enzyme immunoassays for differentiating between the 
      two viruses. We undertook an evaluation of a modified WB assay that includes 
      native HTLV-I viral proteins from MT-2 cells spiked with an HTLV-I transmembrane 
      glycoprotein (recombinant p21e) and the HTLV-I- and HTLV-II-specific recombinant 
      proteins MTA-1 and K55. The test panel consisted of well-characterized sera from 
      U.S. blood donors, American Indians, intravenous drug users, and patients seen in 
      sexually transmitted disease clinics. Of 158 HTLV-I/II-seropositive serum 
      specimens tested, 156 (98.7%) were confirmed and typed as HTLV-I or HTLV-II. Of 
      82 HTLV-I/II-seroindeterminate or -seronegative serum specimens, only 1 was 
      classified as HTLV-II positive: the sample had weak gag p19 and strong gag p24 
      and env p21e reactivity and was radioimmunoprecipitation assay negative for env 
      gp61/68 but polymerase chain reaction positive for HTLV-II. The specificity of 
      the modified WB for confirming and typing serum samples was therefore 100%. We 
      conclude that this WB assay is useful for confirming and typing HTLV infection 
      and can help simplify HTLV-I/II testing algorithms.
FAU - Roberts, B D
AU  - Roberts BD
AD  - Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, 
      Georgia 30333.
FAU - Foung, S K
AU  - Foung SK
FAU - Lipka, J J
AU  - Lipka JJ
FAU - Kaplan, J E
AU  - Kaplan JE
FAU - Hadlock, K G
AU  - Hadlock KG
FAU - Reyes, G R
AU  - Reyes GR
FAU - Chan, L
AU  - Chan L
FAU - Heneine, W
AU  - Heneine W
FAU - Khabbaz, R F
AU  - Khabbaz RF
LA  - eng
GR  - DA06596/DA/NIDA NIH HHS/United States
GR  - HL33811/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (HTLV-II Antibodies)
SB  - IM
MH  - Blotting, Western/*methods/statistics & numerical data
MH  - Evaluation Studies as Topic
MH  - HTLV-I Antibodies/blood
MH  - HTLV-I Infections/diagnosis
MH  - HTLV-II Antibodies/blood
MH  - HTLV-II Infections/diagnosis
MH  - Human T-lymphotropic virus 1/classification/*immunology/isolation & purification
MH  - Human T-lymphotropic virus 2/classification/*immunology/isolation & purification
MH  - Humans
MH  - Sensitivity and Specificity
MH  - Serotyping
PMC - PMC262746
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
PMCR- 1993/02/01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
PHST- 1993/02/01 00:00 [pmc-release]
AID - 10.1128/jcm.31.2.260-264.1993 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1993 Feb;31(2):260-4. doi: 10.1128/jcm.31.2.260-264.1993.