PMID- 8436190
OWN - NLM
STAT- MEDLINE
DCOM- 19930322
LR  - 20061115
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 23
IP  - 2
DP  - 1993 Feb
TI  - Cross-linking of surface IgM, but not surface IgD receptors, by soluble 
      monoclonal antibodies primes murine B cells to secrete immunoglobulin in response 
      to lymphokines.
PG  - 574-7
AB  - We have previously reported the development of a two-step culture system in which 
      soluble anti-mu monoclonal antibodies prime small resting murine B cells to 
      secrete immunoglobulin (Ig) in response to restimulation with a mixture of 
      interleukin-4 (IL-4) and IL-5. Here we have extended these studies to investigate 
      the effects of engaging surface IgD (sIgD). We find that, unlike anti-mu, three 
      different anti-delta monoclonal antibodies did not prime B cells to secrete Ig. 
      In addition, these anti-delta antibodies inhibited anti-mu-stimulated priming for 
      Ig secretion, while enhancing DNA synthesis in response to anti-mu. Furthermore, 
      anti-delta antibodies still inhibited anti-mu-induced priming when added 24-48 h 
      after anti-mu. These results therefore suggest that triggering of sIgD on B cells 
      induces a dominant inhibitory signal which is not necessarily dependent upon 
      co-ligation of sIgM and sIgD receptors. In addition, these findings raise the 
      possibility that ligating sIgM or sIgD receptors on mature B cells in the absence 
      of T cell help, may produce different downstream effects.
FAU - Phillips, C
AU  - Phillips C
AD  - National Institute for Medical Research, Mill Hill, London, GB.
FAU - Klaus, G G
AU  - Klaus GG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Surface)
RN  - 0 (Immunoglobulin delta-Chains)
RN  - 0 (Immunoglobulin mu-Chains)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lymphokines)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (immunoglobulin D receptor)
RN  - 0 (immunoglobulin M receptor)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antigens, Surface/*immunology
MH  - B-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - DNA Replication
MH  - Immunoglobulin delta-Chains/immunology
MH  - Immunoglobulin mu-Chains/immunology
MH  - Immunoglobulins/*biosynthesis
MH  - Lymphocyte Activation/immunology
MH  - Lymphokines/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred CBA
MH  - Receptors, Fc/*immunology
MH  - Receptors, Immunologic/*immunology
MH  - Solubility
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
AID - 10.1002/eji.1830230243 [doi]
PST - ppublish
SO  - Eur J Immunol. 1993 Feb;23(2):574-7. doi: 10.1002/eji.1830230243.