PMID- 8437858 OWN - NLM STAT- MEDLINE DCOM- 19930325 LR - 20211203 IS - 0950-9232 (Print) IS - 0950-9232 (Linking) VI - 8 IP - 3 DP - 1993 Mar TI - Structure, expression and chromosomal mapping of c-akt: relationship to v-akt and its implications. PG - 745-54 AB - Sequence analysis of a nearly full-length murine c-akt cDNA clone and comparison with v-akt revealed the following: (a) The entire coding region of c-akt is identical to that of v-akt with the exception of five G to A transitions that do not alter the reading frame. The 3' untranslated regions of v-akt and c-akt are also identical with the exception of three single-base differences. (b) The recombination event that gave rise to v-akt occurred between the virus at nucleotide 785 from the Gag ATG codon and the 5' untranslated region of c-akt to 60 bp 5' from the c-akt ATG codon. (c) Three nucleotides absent from both Gag and c-akt were inserted at the junction between the two genes. The outcome of these events was to place, in frame, a 63-bp fragment between Gag and Akt. The resulting v-akt oncogene is predicted to encode a tripartite Gag (p12, p15, delta p30)-X-c-akt protein product. The c-akt protein contains, starting from its amino terminus, a src homology 2-like (SH2-like) domain, a domain rich in glutamic acid residues, part of which is predicted to form an amphipathic helix, and a kinase domain encoding a serine-threonine kinase with high degree of homology to members of the protein kinase C (PKC) family. The mouse c-akt is 90% homologous to human AKT1/RAC at the nucleic acid level and 98% homologous at the amino acid level. c-akt in the mouse is composed of 13 exons. The first exon contains a 5' untranslated GC-rich region. Since the recombination that gave rise to v-akt occurred with the 5' untranslated region, we hypothesize that the transduction of c-akt was preceded by provirus insertion upstream from or within the 5' untranslated region and in the same transcriptional orientation as the gene. c-akt was mapped by fluorescence in situ hybridization (FISH) to mouse chromosome 12 and rat chromosome 6 in close proximity to the Igh locus. FAU - Bellacosa, A AU - Bellacosa A AD - Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111. FAU - Franke, T F AU - Franke TF FAU - Gonzalez-Portal, M E AU - Gonzalez-Portal ME FAU - Datta, K AU - Datta K FAU - Taguchi, T AU - Taguchi T FAU - Gardner, J AU - Gardner J FAU - Cheng, J Q AU - Cheng JQ FAU - Testa, J R AU - Testa JR FAU - Tsichlis, P N AU - Tsichlis PN LA - eng SI - GENBANK/X65687 GR - CA-06927/CA/NCI NIH HHS/United States GR - CA-38047/CA/NCI NIH HHS/United States GR - RR-05539/RR/NCRR NIH HHS/United States PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - England TA - Oncogene JT - Oncogene JID - 8711562 RN - 0 (Proto-Oncogene Proteins) RN - 0 (Retroviridae Proteins, Oncogenic) RN - 9007-49-2 (DNA) RN - EC 2.7.11.1 (Akt1 protein, mouse) RN - EC 2.7.11.1 (Akt1 protein, rat) RN - EC 2.7.11.1 (Oncogene Protein v-akt) RN - EC 2.7.11.1 (Protein Serine-Threonine Kinases) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) SB - IM GS - c-akt GS - v-akt MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - *Chromosome Mapping MH - DNA/chemistry MH - Female MH - *Gene Expression MH - Mice MH - Mice, Inbred C57BL MH - Molecular Sequence Data MH - Oncogene Protein v-akt MH - Protein Serine-Threonine Kinases/chemistry/genetics MH - Proto-Oncogene Proteins/chemistry/*genetics MH - Proto-Oncogene Proteins c-akt/chemistry/*genetics MH - *Proto-Oncogenes MH - Rats MH - Retroviridae Proteins, Oncogenic/chemistry/*genetics MH - Transduction, Genetic EDAT- 1993/03/01 00:00 MHDA- 1993/03/01 00:01 CRDT- 1993/03/01 00:00 PHST- 1993/03/01 00:00 [pubmed] PHST- 1993/03/01 00:01 [medline] PHST- 1993/03/01 00:00 [entrez] PST - ppublish SO - Oncogene. 1993 Mar;8(3):745-54.