PMID- 8437863
OWN - NLM
STAT- MEDLINE
DCOM- 19930325
LR  - 20081121
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 8
IP  - 3
DP  - 1993 Mar
TI  - Nasopharyngeal carcinoma shows no detectable retinoblastoma susceptibility gene 
      alterations.
PG  - 791-5
AB  - Since multistage carcinogenesis is frequently associated with the loss of 
      suppressor gene activity, and since in over 90% of cases of nasopharyngeal 
      carcinoma (NPC) p53 alterations are not involved [Sun, Y., Hegamyer, G.H., Cheng, 
      Y.-J., Hildesheim, A., Chen, J.-Y., Chen, I.-H., Cao, Y., Yao, K.-T. & Colburn, 
      N.H. (1992). Proc. Natl. Acad. Sci. USA, 89, 6516-6520] we investigated the 
      possible involvement of the inactivation of the retinoblastoma susceptibility 
      gene (RB) in nasopharyngeal carcinogenesis. We analysed the expression, gross 
      structure and possible point mutation of the RB gene in an NPC cell line as well 
      as seven NPC biopsies obtained from seven patients. The NPC cell line expresses 
      the RB gene with a normal size and abundance, as assayed by reverse transcriptase 
      polymerase chain reaction (RT-PCR) and Northern hybridization. No point mutation 
      was detected in two independent E1A/large T-binding regions, which are the common 
      sites for mutations in the RB gene. NPC biopsies also showed no point mutations 
      at four exon-intron boundaries at which point mutations have been reported in 
      other human carcinomas. The biopsies and cell line had no deletions in the 
      promoter region of the gene and showed no gross deletions or rearrangements. 
      Taken together, we conclude that, in contrast to multistage carcinogenesis 
      leading to human retinoblastoma, osteogenic sarcomas and carcinomas of lung, 
      breast, bladder and prostate, nasopharyngeal carcinogenesis appears unlikely to 
      involve RB gene alterations.
FAU - Sun, Y
AU  - Sun Y
AD  - BCDP, Program Resources Inc./DynCorp, Frederick, Maryland 21702.
FAU - Hegamyer, G
AU  - Hegamyer G
FAU - Colburn, N H
AU  - Colburn NH
LA  - eng
GR  - N01-CO-74102/CO/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Adenovirus E1A Proteins)
RN  - 0 (Antigens, Polyomavirus Transforming)
SB  - IM
GS  - RB
MH  - Adenovirus E1A Proteins/metabolism
MH  - Antigens, Polyomavirus Transforming/metabolism
MH  - Base Sequence
MH  - Carcinoma/*genetics
MH  - Gene Deletion
MH  - Gene Expression
MH  - Gene Rearrangement
MH  - *Genes, Retinoblastoma/genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nasopharyngeal Neoplasms/*genetics
MH  - *Point Mutation
MH  - Promoter Regions, Genetic
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1993 Mar;8(3):791-5.