PMID- 8438167
OWN - NLM
STAT- MEDLINE
DCOM- 19930325
LR  - 20220309
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 259
IP  - 5098
DP  - 1993 Feb 19
TI  - A functional role for GTP-binding proteins in synaptic vesicle cycling.
PG  - 1169-72
AB  - The squid giant synapse was used to test the hypothesis that 
      guanosine-5'-triphosphate (GTP)-binding proteins regulate the local distribution 
      of synaptic vesicles within nerve terminals. Presynaptic injection of the 
      nonhydrolyzable GTP analog GTP gamma S irreversibly inhibited neurotransmitter 
      release without changing either the size of the calcium signals produced by 
      presynaptic action potentials or the number of synaptic vesicles docked at 
      presynaptic active zones. Neurotransmitter release was also inhibited by 
      injection of the nonhydrolyzable guanosine diphosphate (GDP) analog GDP beta S 
      but not by injection of AIF4-. These results suggest that a small molecular 
      weight GTP-binding protein directs the docking of synaptic vesicles that occurs 
      before calcium-dependent neurotransmitter release. Depletion of undocked synaptic 
      vesicles by GTP gamma S indicates that additional GTP-binding proteins function 
      in the terminal at other steps responsible for synaptic vesicle replenishment.
FAU - Hess, S D
AU  - Hess SD
AD  - Department of Biological Sciences, University of Southern California.
FAU - Doroshenko, P A
AU  - Doroshenko PA
FAU - Augustine, G J
AU  - Augustine GJ
LA  - eng
GR  - NS-21624/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Aluminum Compounds)
RN  - 0 (Thionucleotides)
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 21340-02-3 (tetrafluoroaluminate)
RN  - 284SYP0193 (Fluorine)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 71376-97-1 (guanosine 5'-O-(2-thiodiphosphate))
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - CPD4NFA903 (Aluminum)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - Q80VPU408O (Fluorides)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Aluminum/pharmacology
MH  - *Aluminum Compounds
MH  - Animals
MH  - Calcium/metabolism
MH  - Decapodiformes
MH  - *Fluorides
MH  - Fluorine/pharmacology
MH  - GTP-Binding Proteins/*physiology
MH  - Ganglia/physiology
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Guanosine Diphosphate/analogs & derivatives/metabolism/pharmacology
MH  - Guanosine Triphosphate/metabolism
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Models, Neurological
MH  - Nerve Endings/physiology/ultrastructure
MH  - Signal Transduction/drug effects
MH  - Synaptic Vesicles/drug effects/*physiology/ultrastructure
MH  - Thionucleotides/pharmacology
MH  - Time Factors
EDAT- 1993/02/19 00:00
MHDA- 1993/02/19 00:01
CRDT- 1993/02/19 00:00
PHST- 1993/02/19 00:00 [pubmed]
PHST- 1993/02/19 00:01 [medline]
PHST- 1993/02/19 00:00 [entrez]
AID - 10.1126/science.8438167 [doi]
PST - ppublish
SO  - Science. 1993 Feb 19;259(5098):1169-72. doi: 10.1126/science.8438167.