PMID- 8440919
OWN - NLM
STAT- MEDLINE
DCOM- 19930401
LR  - 20190723
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 100
IP  - 3
DP  - 1993 Mar
TI  - Autologous melanoma vaccine induces inflammatory responses in melanoma 
      metastases: relevance to immunologic regression and immunotherapy.
PG  - 335S-341S
AB  - Human primary malignant melanoma is often accompanied by a host response of 
      infiltrating lymphocytes suggestive of tumor antigen-induced immunity and 
      correlated in some tumors with prognosis. Whereas metastatic melanoma deposits 
      typically are not inflamed and contain relatively few lymphocytes and dendritic 
      immune cells, immunization with autologous melanoma-cell vaccine may induce a 
      clinical inflammatory response associated with mononuclear-cell infiltration. In 
      this study, we characterize immune responses to dermal and subcutaneous melanoma 
      metastases in dinitrophenyl (DNP)-pre-sensitized patients immunized with 
      DNP-conjugated melanoma cells. Patients so treated develop cutaneous delayed 
      hypersensitivity responses to DNP-conjugated autologous mononuclear cells, and 
      approximately one-half show clinical evidence of inflammation and regression of 
      metastases within 2-4 months. Whereas pre-vaccination biopsies of metastatic 
      melanoma failed to reveal significant infiltration by lymphocytes, biopsies 
      obtained after vaccination and coincident with clinical inflammation were 
      markedly infiltrated preponderantly by T cells with a CD8+ phenotype. Clustering 
      of these cells about individual degenerating melanoma cells in a manner analogous 
      to "satellitosis" was a consistent feature of this reaction. Enhanced expression 
      of intercellular adhesion molecule-1 (ICAM-1) and human leukocyte antigen 
      (HLA)-DR by melanoma cells were invariably associated with zones of T-cell 
      infiltration, whereas diminished or absent expression was observed in relatively 
      unaffected regions of tumors. Numerous HLA-DR+, CD4+, CD1-, Leu-1- dendritic 
      cells were also associated with zones of early T-cell infiltration. These data 
      indicate that clinical inflammation and regression of metastatic melanoma induced 
      by autologous melanoma-cell vaccine involves activated T cells with 
      cytotoxic-suppressor phenotype and dendritic cells putatively capable of local 
      antigen presentation. ICAM-1 upregulation on melanoma cells is a likely mediator 
      of ligand interaction between infiltrating T cells and target cells in this model 
      of antigen-induced host anti-tumor response. Structural alterations identified in 
      this setting (e.g., tumor cell satellitosis) may provide additional insight into 
      identifying features of naturally occurring host immune responses to primary 
      cutaneous melanomas.
FAU - Murphy, G F
AU  - Murphy GF
AD  - Department of Dermatology, University of Pennsylvania School of Medicine, 
      Philadelphia.
FAU - Radu, A
AU  - Radu A
FAU - Kaminer, M
AU  - Kaminer M
FAU - Berd, D
AU  - Berd D
LA  - eng
GR  - CA-39248/CA/NCI NIH HHS/United States
GR  - CA-40358/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Vaccines)
SB  - IM
MH  - Dermatitis, Atopic/*etiology
MH  - Humans
MH  - Hypersensitivity, Delayed/etiology
MH  - Immunotherapy
MH  - Inflammation/*etiology
MH  - Lymphatic Metastasis
MH  - Lymphocytes, Tumor-Infiltrating
MH  - Melanoma/immunology/*secondary/therapy
MH  - Skin Neoplasms/immunology/*secondary/therapy
MH  - Time Factors
MH  - Vaccines/adverse effects
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
AID - 10.1111/1523-1747.ep12470236 [doi]
PST - ppublish
SO  - J Invest Dermatol. 1993 Mar;100(3):335S-341S. doi: 10.1111/1523-1747.ep12470236.